Modified tert promoter with enhanced tumor-specificity and...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S320100, C424S093100, C514S04400A

Reexamination Certificate

active

08067567

ABSTRACT:
The present invention relates to a transcriptional regulatory sequence with enhanced tumor-specificity and strength and a recombinant vector comprising the transcriptional regulatory sequence. More particularly, the present invention relates to a transcriptional regulatory sequence comprising a human telomere reverse transcriptase (hTERT) promoter linked to a nucleotide sequence that comprises one or more c-Myc binding sites and/or one or more Sp1 binding sites, and a recombinant vector comprising a certain gene that is operably linked to the above transcriptional regulatory sequence.

REFERENCES:
patent: 7491525 (2009-02-01), Qian et al.
patent: 00/03104 (2000-08-01), None
patent: WO 03/006640 (2003-01-01), None
patent: WO 03/013555 (2003-02-01), None
pXC1 vector map, 1 page, printed from Microbix Biosystems website, Nov. 20, 2010.
pXC1 vector DNA sequence, 4 pages, printed from Microbix Biosystems website, Nov. 20, 2010.
Supplementary European Search Report, dated Jun. 29, 2006, for EPO Application No. EP04715577.
Kirch et al., “Tumor-specific activation of hTERT-derived promoters by tumor suppressive E1A-mutants involves recruitment of p300/CBP/HAT and suppression of HDAC-1 and defines a combined tumor targeting and suppression system” Oncogene, vol. 21, No. 52, Nov. 14, 2002, pp. 7991-8000.
Wick et al., “Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT)”, Gene: An International Journal on Genes and Genomes, Elsevier, Amsterdam, NL, vol. 232, May 17, 1999, pp. 97-106.
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Kyo, S. et al., “Sp1 cooperates with c-Myc to activate transcription of the human telomerase reverse transcriptase gene (hTERT)” Nucleic Acids Research, vol. 28, No. 3, pp. 669-677, 2000.
Takakura, M. et al., “Cloning of Human Telomerase Catalytic Subunit (hTERT) Gene Promoter and Identification of Proximal Core Promoter Sequences Essential for Transcriptional Activation in Immortalized and Cancer Cells” Cancer Research, vol. 59, pp. 551-557, Feb. 1999.
Horikawa, I. et al., “Cloning and Characterization of the Promoter Region ofHuman Telomerase Reverse TranscriptaseGene” Cancer Research, vol. 59, pp. 826-830, Feb. 1999.
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Abdallah, B. et al., “Non-viral gene transfer: Applications in developmental biology and gene therapy” Biology of the Cell, vol. 85, pp. 1-7, 1995.
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