Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-04-18
2006-04-18
Rao, Manjunath (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S004000, C435S006120, C435S252300, C435S254300, C435S320100, C435S440000, C536S023200, C536S023740
Reexamination Certificate
active
07029888
ABSTRACT:
The present invention relates to a modified enzyme of a non-heme iron (II) dependent family of oxygenases and oxidases which renders the enzyme dependent on bicarbonate for activity. In a preferred embodiment, the modification is an arginine, lysine, or other amino acid that is two amino acid residues upstream of a histidine residue that is an iron ligand in the enzyme and is one of the histidine residues of the 2-histidine-1-aspartic acid trifacial motif. In particular, the modified enzymes are isopenicillin N synthetase, deacetoxycephalosporin C synthetase, and deacetoxycephalosporin C synthetase/deacetylcephalosporin C synthetase which are used to make antibiotics. The present invention further provides a method for making antibiotics using a modified enzyme such as isopenicillin N synthetase, deacetoxycephalosporin C synthetase, and deacetoxycephalosporin C synthetase/deacetylcephalosporin C synthetase wherein the modification renders the enzyme dependent on bicarbonate for activity. Finally, the present invention provides a method for making antibiotics by providing the modified enzyme either in either an organism or in a cell-free system.
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Dilley David R.
Kadyrzhanova Dina K.
Wang Zhenyong
Warner Toni M.
Board of Trustees of Michigan State University
McLeod Ian C.
Pak Yong
Rao Manjunath
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