Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-07
1999-09-21
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 4351723, 435220, 435222, 43525231, 4353201, 510300, 536 232, 935 14, 935 29, 935 74, C12N 952, C12N 956, C12N 1557, C12N 1575
Patent
active
059553405
ABSTRACT:
Novel carbonyl hydrolase mutants derived from the amino acid sequence of naturally-occurring or recombinant non-human carbonyl hydrolases and DNA sequences encoding the same. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to encode the substitution, insertion or deletion of one or more amino acids in the amino acid sequence of a precursor carbonyl hydrolase. Such mutants have one or more properties which are different than the same property of the precursor hydrolase.
REFERENCES:
patent: Re34606 (1994-05-01), Estell et al.
patent: 4002572 (1977-01-01), te Nijenhuis
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4543329 (1985-09-01), Daum et al.
patent: 4760025 (1988-07-01), Estell et al.
Rastetter, W.H., "Enzyme engineering: applications and promise." Trends Biotechnol., 1:80-84 (1983).
Winter, G., et al., "Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding." Nature, 299:756-758 (1982).
Wallace, B.R., et al., "Oligonucleotide directed mutagenesis of the human .beta.-globin gene: a general method for producing specific point mutations in cloned DNA." Nucleic Acids Res., 9:3647-3656 (1981).
Shortle, D., et al., "Gap misrepair mutagenesis: Efficient site-directed induction of transition, transversion, and frameshift mutations in vitro." Proc. Natl. Acad. Sci. USA., 79:1588-1592 (1982).
Wells, J.A., "Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis." Nucleic Acids Res., 11:8913-8926 (1985).
Vasantha, N., et al., "Genes for Alkaline Protease and Neutral Protease from Bacillus amyloliquefaciens Contain a Large Open Reading Frame Between the Regions Coding for Signal Sequence and Mature Protein." J. Bacteriol., 159:811-819 (1984).
Jacobs, M., et al., "Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis." Nucleic Acids Res., 13: 8913-8926 (1985).
Stahl, M.L., et al., "Replacement of the Bacillus subtilis Subtilisin Structural Gene with and In Vitro-Derived Deletion Mutation." J. Bacteriol., 158:411-418 (1984).
Svendsen, I., "Chemical Modifications of the Subtilisins with Special Reference to the Binding of Large Substrates. A Review." Carlsberg Res. Commun., 41:237-291 (1976).
Smith, E.L., "The Complete Sequence; Comparison with Subtilisin BPN; Evolutionary Relationships." J. Biol. Chem., 243:2184-2191 (1968).
Kurihara, M., et al., "Isolation and Sequence of the Chymotryptic Peptides and the Complete Amino Acid Sequence." J. Biol. Chem., 247:5619-5631 (1972).
Svendsen, I., et al., "Complete Amino Acid Sequence of Alkaline Mesentericopeptidase: A subtilisin isolate from a strain of Bacillus mesentericus." FEBS, 196:228-232 (1986).
Wright, C.S., "Structure of Subtilisin BPN' at 2.5 A Resolution." Nature, 221:235-242 (1969).
Grantham, R., "Amino Acid Difference Formula to Help Explain Protein Evolution." Science, 185:962-964 (1974).
Stauffer, C.E., et al., "The Effect on Subtilisin Activity of Oxidizing a Methionine Residue." J. Biol. Chem. 244:5333-5338 (1969).
Jaye, M., et al., "Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX." Nucleic Acids Res., 11:2325-2335 (1983).
Cantor, C.R., et al. "Biophysical Chemistry: Part I: The conformation of biological macromolecules." W.H. Freeman and Company, San Francisco, pp. 3-154, 253-309 (1980).
Robertus, J.D., et al., "An X-Ray Crystallographic Study of the Binding of Peptide Chloromethyl Ketone INhibitors to Subtilisin BPN'," Biochemistry, 11:2439-2449 (1972).
Ricchelli, F., et al., "Effects of pH and Urea on the Conformational Properties of Subtilisin DY." Biochemical J., 207:201-205 (1982).
Mattoccia, E., et al., "Mutation in the A Block of the Yeast tRNA.sup.Leu.sub.3 Gene that Allows Transcription but Abolishes Splicing and 5'-End Maturation," Cell, 32:67-76 (1983).
Inouye, S., et al., "Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane." Proc. Natl. Acad. Sci. USA, 79:3438-3441 (1982).
Inouye, S., et al., "Effects of Mutations at Glycine Residues in the Hydrophobic Region of the Escherichia coli Prolipoprotein Signal Peptide on the Secretion across the Membrane." J. Biol. Chem., 259:3729-3733 (1984).
Carter, P., et al., "Improved Oligonucleotide Site-Directed Mutagenesis Using M13 Vectors." Nucleic Acids Res. , 13:4431-4443 (1985).
Baughman, G., et al., "Translational regulation of the L11 ribosomal protein operon of Escherichia coli: Analysis of the mRNA target site using olignucleotide-directed mutagenesis." Proc. Natl. Acad. Sci. USA, 81:5389-5393 (1984).
Carter, P.J., et al., "The Use of Double Mutants to Detect Structural Changes in the Active Site of the Tyrosyl-tRNA Synthetase (Bacillus stearothermophilus)." Cell, 33:835-840 (1984).
Stroud, R.M., Sci Amer., 131:74-88 (1974).
Kraut, J., Ann. Rev. Biochem., 46:331-358 (1977).
Markland, F.S., et al., The Enzymes, ed. Boyer, P.D., Acad Press, New York, vol. III, pp. 561-608 (1971).
Nedkov, P., et al., Hoppe-Seyler's Z. Physiol. Chem., 364:1537-1540 (1983).
Drenth, J., et al., Eur. J. Biochem., 26, 177-181 (1972).
Matthews, D.A., et al., J. Biol. Chem., 250:7120-7126 (1975).
Poulos, T.L., et al., J. Biol. Chem., 251:1097-1103 (1976).
Philipp, M., et al., Mol. Cell. Biochem., 51:5-32 (1983).
Jencks, W.P., Catalysis in Chemistry and Enzymology, McGraw-Hill, pp. 321-436 (1969).
Fersht, A., Enzyme Structure and Mechanism, Freeman, San Francisco, pp. 226-287 (1977).
Kaiser, E.T., et al., Ann. Rev. Biochem., 54:565-595 (1985).
Kraut, J., Ann. Rev. Biochem., 46:331-358 (1977).
Ulmer, K.M. Science, 219:666-671 (1983).
Wilkinson, A.J., et al., Biochemistry, 22:3581-3586 (1983).
Wilkinson, A.H., et al., Nature, 307:187-188 (1984).
Perry, L.J., et al., Science, 226:555-557 (1984).
Villafranca, D.E., et al., Science, 222:782-788 (1983).
Carter, P.J., et al., Cell, 38:835-840 (1984).
Craik, C.S., et al., Science, 228:291-297 (1985).
Polgar, L. and Sajgo, M., Biochem. Biophy. Acta., 667:351-354 (1981).
Paterson, A. and Clarke, P.H., J. Gen. Micro. 114:65-85 (1979).
Uehara, H., et al., J. Bacteriology, 139:583-590 (1979).
Kerjan, P., et al., Eur. J. Biochem., 98:353-362 (1979).
Wells, et al., Nucleic Acids Res., 11:7911-7923 (1983).
Thomas, et al., Nature, 318:37-38 (1985).
Gardell, et al., Nature, 317:551-555 (1985).
Vasantha, et al., J. Bacteriol., 165:837-842 (1986).
Zaghloul, et al., J. Bacteriol., 164:550-555 (1985).
Carter, et al., Science, 237:394-399 (1987).
Wells, et al., Cold Spring Harbor Symposia on Quantitative Biology, LII:647-652 (1987).
Toney et al., Science, 243:1485-1488 (1989).
Iverson, et al., Science, 243:1184-1188 (1989).
Wells, J.A., et al., "Designing Substrate Specificity by Protein Engineering of Electrostatic Interactions." Proc. Nat. Acad. Sci. USA, 84:1219-1223 (1987).
Estell, D.A., et al., "Probing Steric and Hydrophobic Effects on Enzyme-Substrate Interactions by Protein Engineering." Science, 233:659-663 (1986).
Wells, J.A., et al., "Protein Engineering of Subtilisin." Protein Engineering, pp. 279-287 (1987).
Abrahmsen, L., et al., "Engineering Subtilisin and Its Substrates for Efficient Ligation of Peptide Bonds in Aqueous Solution." Biochemistry, 30:4151-4159 (1991).
Markland, F.S., Carlsberg Res. Comm., 41:237-291 (1976).
Polgar, et al., Adv. Enzymol., 33:381-400 (1970).
Ottesen, et al., Meth. Enzymol., XIX:199-215 (1970).
Watson, J.D., in Molecular Biology of the Gene, 3rd Edition. Benjamin/Cummings Publ. Co., Inc. Menlo Park, CA p. 313 (1987).
Singleton, et al., Dictionary of Microbiology and Molecular Biology, Second Edition. John Wiley and Sons. Chichester. p. 741 (1987).
Wells, et al., Subtilisin: An Enzyme Designed to be Engineered, in Proteins: Form and Function (Bradshaw et al., eds.). Elsevier Trends Journals, Cambridge, UK, pp. 45-57 (1990).
Allinger, et al., in Organic Chemistry, Worth Publishers, New York, N
Bott Richard Ray
Caldwell Robert Mark
Cunningham Brian C.
Estell David Aaron
Power Scott Douglas
Genencor International Inc.
Moore William W.
Silva Robin M.
Trecartin Richard F.
Wax Robert A.
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