Stock material or miscellaneous articles – Coated or structually defined flake – particle – cell – strand,... – Particulate matter
Patent
1991-09-24
1994-06-07
Nakarani, D. S.
Stock material or miscellaneous articles
Coated or structually defined flake, particle, cell, strand,...
Particulate matter
2101982, 210656, 427215, 428404, 502401, G01N 3048
Patent
active
053188489
DESCRIPTION:
BRIEF SUMMARY
DESCRIPTION
1. Technical Field
This invention relates to a packing material for chromatography and in particular, to an improvement of a surface modifying agent.
The present packing material, especially can be used for separation and analysis of polycyclic aromatic compounds and related compounds.
2. Background Art
Chromatography has been employed for separation and purification of various chemical components.
In such chromatography, as the packing material for adsorption chromatography in which mutual separation between the sample molecules is accomplished through the bipole-bipole interaction, .pi. electron - .pi. electron interaction or hydrogen bond formation force between the sample molecules (or analyzed molecule) and the packing material surface, the whole porous silica gel having macropores and micropores distributed in a network or the porous polymeric material obtained by copolymerization of styrene, etc. has been employed. Particularly, the packing material of a porous silica gel surface modified with a silane coupling agent is different in separation characteristics of sample molecules depending on the structure of the silane coupling agent employed, and therefore useful for efficient separation of sample molecules having various structures.
Incidentally, as the surface modifying agent of the surface-modified silica gel to be used as the packing agent for chromatography, the octadecylsilyl derivative in most widely used, and also butylsilyl derivative, octylsilyl derivative, etc. have been employed.
However, all of these have only one functional group for silylation capable of bonding to the silica gel surface in one molecule, and therefore in a developing solvent for chromatography, functional groups which influence separation characteristics such as the octadecyl group, butyl group, octyl group at the interface between the filler and the solvent exist in disordered form on the silica gel surface. Further, the octadecyl group, butyl group, octyl group, etc. have carbon chains constituting the molecules that are freely rotatably, and therefore there is no homogeneity in the adsorption form when sample molecules are practically adsorbed onto the packing material surface. Such absence of homogeneity is a crucial defect in designing the surface modifying agent to obtain the most suitable stationary phase depending on the structure of sample molecules.
The present invention has been accomplished in view of the problem of the prior art as mentioned above, and its object is to provide a packing material for chromatography that can separate various substances, and further can efficiently be useful for separation and purification within a short time.
DISCLOSURE OF INVENTION
For accomplishing the above object, the present inventors have studied intensively, and consequently found that excellent separation characteristics can be exhibited by having the organic functional groups regularly arranged on the surface of base material for the packing material by use of an aromatic compound having a plurality of functional groups for silylation in one molecule (hereinafter called "multi-legged type silane coupling agent") as the surface modifying agent, to accomplish the present invention.
Accordingly, the present invention provides a packing material for chromatography characterized by modifying the surface of base material of the packing material with a multi-legged type silane coupling agent.
Further, a method for preparation of the packing material for chromatography that comprises reacting a multi-legged type silane coupling agent having a chlorine atom or lower alkoxy group as a- reactive group to a porous silica gel with the silica gel.
Such packing material has an excellent stability and separation characteristics, since aromatic compound moiety constructed of the present silane coupling agent is immobilized on the surface of the base material through the multi-legged type functional groups (a plurality of functional groups).
BRIEF DESCRIPTION OF DRAWINGS
FIG. 1 is a .sup.13 C-solid state high res
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patent: 4746572 (1988-05-01), Glajch
patent: 4782040 (1988-11-01), Revis et al.
patent: 4828695 (1989-05-01), Yamamura et al.
patent: 4835058 (1989-05-01), Komiya et al.
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Journal of Chromatography, 527 (1990) Sep. 26, pp. 193-207.
Itoh Kenji
Jinno Kiyokatsu
Nagashima Hideo
Ohtsu Yutaka
Nakarani D. S.
Shiseido Company Ltd.
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