Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-08-22
1991-02-19
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435 6952, 435 694, 435 698, 435 697, 4351723, 435 91, 4352523, 43525231, C12P 2100, C12N 1500, C12N 700, C07H 1512
Patent
active
049943790
ABSTRACT:
The invention discloses modified signal peptides derived from wild-type signal peptides of the type that are capable of forming membrane-bound lipoproteins and methods for making such modified signal peptides and DNA sequences encoding them. Modified signal peptides of the invention and DNA sequences encoding them are useful for increasing the secretion of heterologous gene products produced by transformed host organisms. The invention further discloses a method for producing recombinant DNA sequences in vivo.
REFERENCES:
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Nielsen et al., J. Biol. Chem., vol. 257(8) Apr. 25, 1982, pp. 4490-4495, "Membrane-band Penicillinases in Gram-positive Bacteria".
Horowicz et al., Nucleic Acids Rev., vol. 9(13) 1981, "Rapid and Efficient Cosmid Cloning".
Lai et al., Proc. Natl. Acad. Sci., vol. 78(6), pp. 3506-3510, Jun. 1981, "Bacillus licheniformis Penicillinase Synthesized in Escherichia coli Contains Covalently Linked Fatty Acid and Glyceride".
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Mezes et al., J. Biol. Chem., vol. 258(18) Sep. 25, 1983, "Construction of penP.DELTA.I, Bacillus licheniformis 749/C .beta.-Lactamase Lacking Site for Lipoprotein Modification".
Inouye et al, EMBO J. vol. 2(1) pp. 87-91, 1983, "Proliprotein Signal Peptidase of Escherichia coli Requires a Cysteine Residue at the Cleavage Site".
Nielsen et al., Proc. Natl. Acad. Sci., vol. 78(6) pp. 3511-3515, Jun. 1981, "Lipoprotein Nature of Bacillus licheniformis Membrane Penicillinase".
Vlasuk et al., J. Biol. Chem., vol. 258(11), Jun. 10, 1983, pp. 7141-7148, "Effects of the Complete Removal of Basic Amino Acid Residues from the Signal Peptide on Secretion of Lipoprotein in Escherichia coli".
Zweibel et al, J. Bacteriology, Jan. 1981, pp. 654-656, vol. 145(1), "Preferential Selection of Deletion Mutations of the Outer Membrane Lipoprotein Gene of Escherichia coli by Globomycin".
Cetus Corporation
Fineman Elliott L.
Giotta Gregory J.
Halluin Albert P.
Teskin Robin L.
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