Modified sarcosine oxidases, genes and recombinant DNAs...

Details Modified sarcosine oxidases, genes and recombinant DNAs... Modified sarcosine oxidases, genes and recombinant DNAs...

2007-03-13

2007-03-13

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C536S023200, C435S252300, C435S252310

Reexamination Certificate

active

10829427

ABSTRACT:
(1) A modified sarcosine oxidase with improved stability in the acidic range compared to a wild-type sarcosine oxidase. (2) A sarcosine oxidase gene encoding a modified sarcosine oxidase of the following (a), (b), or (c):(a) protein composed of the amino acid sequence represented by SEQ ID NO: 1(b) protein composed of an amino acid sequence wherein one or some amino acid(s) are deleted, substituted, or added from the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity(c) protein composed of an amino acid sequence which shows 80% or more homology to the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activityAccording to the present invention, sarcosine oxidases, in particular sarcosine oxidases which show optimal pH and high activity in the slightly acidic range and have improved stability can be prepared efficiently, thus making the invention industrially useful.

REFERENCES:
Nishiya Y. et al. Analysis of Interaction between the Arthrobacter Sarcosine Oxidase and the Coenzyme Flavin Adenine Dinucleotide by Site-Directed Mutagenesis, Applied and Environmental Microbiology, 1996, 62 (7), 2405-2410.
Nishiya Y. A Mutnt Sarcosine Oxidase in Which Activity Depends on Flavin Adenine Dinuclotide, Protein Expression and Purifiction, 2000, 20, 95-97.

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