Modified sarcosine oxidases, genes and recombinant DNAs...

Details Modified sarcosine oxidases, genes and recombinant DNAs... Modified sarcosine oxidases, genes and recombinant DNAs...

2008-05-20

2008-05-20

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S320100, C435S252300, C435S069100, C536S023200

Reexamination Certificate

active

07374918

ABSTRACT:
An isolated DNA molecule encoding modified sarcosine oxidases having optimal pH, high activity in the slightly acidic range and improved stability and a method for preparing the modified sarcosine oxidases is disclosed.

REFERENCES:
patent: 7132253 (2006-11-01), Furukawa et al.
patent: 7189548 (2007-03-01), Furukawa et al.
patent: 2005/0026265 (2005-02-01), Furukawa et al.
Y. Nishiya, “A Mutant Sarcosine Oxidase in Which Activity Depends on Flavin Adenine Dinucleotide”, Protein Expression and Purification, vol. 20, pp. 95-97, 2000.
Y. Nishiya, et al., “Analysis of Interaction Between the Arthrobacter Sarcosine Oxidase and the Coenzyme Flavin Adenine Dinucleotide by Site-Directed Mutagenesis”, Applied and Environmental Microbiology, vol. 62, No. 7, pp. 2405-2410, Jul. 1996.

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