Chemistry: molecular biology and microbiology – Vector – per se
Patent
1996-09-20
2000-02-15
Degen, Nancy
Chemistry: molecular biology and microbiology
Vector, per se
435 6, 4351721, 4351723, 536 231, 536 235, 536 241, C12N 1563, C12N 1511, C07H 2104
Patent
active
060251921
ABSTRACT:
The present invention relates to methods and compositions for the elucidation of mammalian gene function. Specifically, the present invention relates to methods and compositions for improved mammalian complementation screening, functional inactivation of specific essential or non-essential mammalian genes, and identification of mammalian genes which are modulated in response to specific stimuli.
In particular, the compositions of the present invention include, but are not limited to, replication-deficient retroviral vectors, libraries comprising such vectors, retroviral particles produced by such vectors in conjunction with retroviral packaging cell lines, integrated provirus sequences derived from the retroviral particles of the invention and circularized provirus sequences which have been excised from the integrated provirus sequences of the invention. The compositions of the present invention further include novel retroviral packaging cell lines.
REFERENCES:
patent: 4959317 (1990-09-01), Sauer
patent: 5378618 (1995-01-01), Sternberg et al.
Blasina, A. et al., 1996, "Copy-Up Mutants of the Plasmid RK2 Replication Initiation Protein are Defective in Coupling RK2 Replication Origins", Proc. Natl. Acad. Sci USA 93:3559-3564.
Cheng, L. et al., 1996, "Use of Green Fluorescent Protein Variants to Monitor Gene Transfer and Expression in Mammalian Cells", Nature Biotechnol. 14:606-609.
Choulika, A. et al., 1996, "Transfer of Single Gene-Containing Long Terminal Repeats into the Genome of Mammalian Cells by a Retroviral Vector Carrying the cre Gene and the IoxP Site", J. Virol. 70:1792-1798.
Colas, P. et al., 1996, "Genetic Selection of Peptide Aptamers that Recognize and Inhibit Cyclin-Dependent Kinase 2", Nature 380:548-550.
Levy, J. et al., 1996, "Retroviral Transfer and Expression of a Humanized, Red-Shifted Green Fluorescent Protein Gene into Human Tumor Cells", Nature Biotechnol. 14:610-614.
Socolic, R. et al., 1996, "A Bicistronic Retrovirus Vector Containing a Picornavirus Internal Ribosome Entry Site Allows for Correction of X-Linked CGD by Selection for MDR1 Expression", Blood 87:42-50.
Suzuki, H. et al., 1996, "Infection of Human Cells by Murine Ecotropic Viruses: Retroviral Vectors Carrying the Hygromycin Resistance-Encoding Gene", Gene 170:255-259.
Bergemann, J. et al., 1995, "Excision of Specific DNA-Sequences from Integrated Retroviral Vectors via Site-Specific Recombination", Nucl. Acids Res. 23:4451-4456.
Casini, T. and Graf, T., 1995, "Bicistronic Retroviral Vector Reveals Capacity of v-erbA to Induce Erythroleukemia and to Co-Operate with v-myb", Oncogene 11:1019-1026.
Gunnery, S. Mathews, M., 1995, "Functional mRNA Be Generated by RNA Polymerase III", Mol. Cell. Biol. 15:3597-3607.
Hsieh, C. et al., 1995, "Improved Gene Expression by a Modified Bicistronic Retroviral Vector", Biochem. Biophys. Res. Comm. 214:910-917.
Meyer, K. et al., 1995, "Interaction of Eukaryotic Initiation Factor eIF-4B with a Picornavirus Internal Translation Initiation Site", J. Virol. 69:2819-2824.
Price, J. et al., 1995, "The Expression of Functional Genes in the Rodent Nervous System with Dual-Expressing Retroviral Vectors Encoding an IRES Sequence", J. Cell. Biochem. Suppl O (21A):413.
Shah, D. et al., 1995, "Dissection of the Core and Auxiliary Sequences in the Vegetative Replication Origin of Promiscuous Plasmid RK2", J. Mol. Biol. 254:608-622.
Sugimoto, Y. et al., 1995, "Co-Expression of a Multidrug Resistance Gene (MDR1) and HSV-TK Gene in a Bicistronic Retroviral Vector pHa-MDR-IRES-TK Allows Selective Killing of MDR1-transduced Human Tumor Transplanted in Nude Mice", Proc. Amer. Assoc. for Cancer Res. 36:413.
Sugimoto, Y. et al., 1995, "Retroviral Coexpression of a Multidrug Resistance Gene (MDR1) and Human .alpha.-Galactosidase A for Gene Therapy of Fabry Disease", Hum. Gene Ther. 6:905-915.
Vagner, S. et al., 1995, "Alternative Translation Initiation of the Moloney Murine Leukemia Virus mRNA Controlled by Internal Ribosome Entry Involving the p57/PTB Splicing Factor", J. Biol. Chem. 270:20376-20383.
Vile, R. and Russell, S., 1995, "Retroviruses as Vectors", Brit. Med. Bull. 51:12-30.
Whitehead, I. et al. 1995, "Expression Cloning of Oncogenes by Retroviral Transfer of cDNA Libraries", Mol. Cell. Biol. 15:704-710.
Whitehead, I. et al., 1995, "Retroviral Transduction and Oncogenic Selection of a cDNA Encoding Dbs, a Homolog of the Dbl Guanine Nucleotide Exchange Factor", Oncogene 10:713-721.
Whitehead, I. et al., 1995, "Retroviral-Mediated Expression Cloning of a Novel Oncogene with Structural Similarities to the CDC24 Family of Guanine Nucleotide Exchange Factors", J. Cell. Biochem. Suppl. O (19A):45.
Whitehead, I. et al., 1995, "Expression Cloning of Ifc, a Novel Oncogene with Structural Similarities to Guanine Nucleotide Exchange Factors and to the Regulatory Region of Protein Kinase C", J. Biol. Chem. 270:18388-18395.
Bernard, P. et al., 1994, "Positive-Selection Vectors Using the F Plasmid ccdB Killer Gene", Gene 148:71-74.
Qin, M et al., 1994, Cre Recombinase-Mediated Site-Specific Recombination Between Plant Chromosomes, Proc. Natl. Acad. Sci. USA 91:1706-1710.
Rayner, J. and Gonda, T., 1994, "A Simple and Efficient Procedure for Generating Stable Expression Libraries by cDNA Cloning in a Retroviral Vector", Mol. Cell. Biol. 14:880-887.
Scheper, G. ., 1994, "Binding of Eukaryotic Initiation tor-2 and trans-Acting Factors to the 5' Untranslated Region of Encephalomyocarditis Virus RNA", Biochimie 76:801-809.
Soares, M. et al., 1994, "Construction and Characterization of a Normalized cDNA Library", Proc. Natl. Acad. Sci. USA 91:9228-9232.
Sugimoto, Y. et al., 1994, "Efficient Expression of Drug-Selectable Genes in Retroviral Vectors Under Control of an Internal Ribosome Entry Site", Bio/Technology 12:694-698.
Tramontano, A. et al., 1994, "The Making of the Minibody: An Engineered .beta.-Protein for the Display of Conformationally Constrained Peptides", J. Mol. Recognition 7:9-24.
Palmer, T. et al., 1993, "Efficient Expression of a Protein Coding Gene Under the Control of an RNA Polymerase I Promoter", Nucl. Acids Res. 21:3451-3457.
Pear, W. et al., 1993, "Production of High Titer Helper-Free Retroviruses by Transient Transfection", Proc. Nat'l. Acad. Sci USA 90:8392-8396.
Haller, A. and Semler, B., 1992, "Linker Scanning Mutagenesis of the Internal Ribosome Entry Site of Poliovirus RNA", J. Virol. 66:5075-5086.
Morgan, R. et al., 1992, "Retroviral Vectors Containing Putative Internal Ribosome Entry Sites: Development of a Polycistronic Gene Transfer System and Applications to Human Gene Therapy", Nucl. Acids Res. 20:1293-1299.
Rice, G. et al., 1992, "Random PCR Mutagenesis Screening of Secreted Proteins by Direct Expression in Mammalian Cells", Proc. Natl. Acad. Sci USA 89:5467-5471.
Cook, R. et al., 1991, "Retrotransposon Gene Engineering", Bio/Technology 9:748-751.
Ghattas, I. et al., 1991, "The Encephalomyocarditis Virus Internal Ribosome Entry Site Allows Efficient Coexpression of Two Genes From a Recombinant Provirus in Cultured Cells and Embryos", Mol. Cell. Biol. 11:5848-5859.
Jorgensen, P. et al., 1991, "Tagging the Genome of the Murine Leukemia Retrovirus SL3-3 by a Bacterial Iac Operator Sequence", Gene 109:243-248.
McLachlin, J. et al., 1990, "Retroviral-Mediated Gene Transfer", Prog. Nucl. Acid Res. and Mol. Biol. 38:91-135.
Morgenstern, J. and Land, H., 1990, "Advanced Mammalian Gene Transfer: High Titre Retroviral Vectors with Multiple Drug Selection Markers and a Complementary Helper-Free Packaging Cell Line", Nucl. Acids Res. 18:3587-3596.
Ott, D. et al., 1990, "Sequence Analysis of Amphotropic and 10A1 Murine Leukemia Viruses: Close Relationship to Mink Cell Focus-Inducing Viruses", J. Virol. 64:757-766.
Tolstoshev, P. and Anderson, W., 1990, "Gene Expression Using Retroviral Vectors", Curr. Opinion in Biotech. 1:55-61.
Danos, O. and Mulligan, R., 1988, "Safe and Efficient Generation of Recombinant Retroviruses with Amphotrophic and Ectrophic Host Ranges", Proc. Natl. Acad. Sci. USA 85:6460-6464.
Eglitis, M. and Anderson, W., 1988, "Retroviral Vectors Introd
Beach David
Hannon Gregory J.
Cold Spring Harbor Laboratory
Degen Nancy
McGarry Sean
Steel Diana M.
Vincent Matthew P.
LandOfFree
Modified retroviral vectors does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Modified retroviral vectors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified retroviral vectors will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1905061