Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-11-14
2006-11-14
Saidha, Tekchand (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S440000, C435S004000, C435S006120, C435S069100, C435S025000, C536S023200, C536S023700
Reexamination Certificate
active
07135318
ABSTRACT:
There are disclosed a reductase having good heat stability, which resulted in decrease in reaction time and improvement of reaction efficiency. Specifically, disclosed is an enzyme having an amino acid sequence of SEQ ID NO:1 in which at least one of the amino acids of the positions 245 and 271 of the amino acid sequence of SEQ ID NO:1 is replaced with another amino acid(s); a polynucleotide having a nucleotide sequence that encodes the amino acid sequence of said reductase; a vector containing said polynucleotide; a transformant containing said polynucleotide or said vector, and the like.
REFERENCES:
patent: 4455373 (1984-06-01), Higgins
patent: 4895979 (1990-01-01), Noyori et al.
patent: 5215919 (1993-06-01), Miya et al.
patent: 5233095 (1993-08-01), Fellmann et al.
patent: 5908953 (1999-06-01), Matsuda et al.
patent: 6218156 (2001-04-01), Yasohara et al.
patent: 6312933 (2001-11-01), Kimoto et al.
patent: 2003/0134402 (2003-07-01), Asako et al.
patent: 2003/0186400 (2003-10-01), Asako et al.
patent: 0 400 239 (1990-12-01), None
patent: 0 967 271 (1999-12-01), None
patent: 1 013 758 (2000-06-01), None
patent: 1 201 647 (2002-05-01), None
patent: 1 213 354 (2002-06-01), None
patent: 60-251890 (1985-12-01), None
patent: 63-123387 (1988-05-01), None
patent: 01-222787 (1989-09-01), None
patent: 02-312593 (1990-12-01), None
patent: 2532299 (1996-06-01), None
patent: 2566962 (1996-10-01), None
patent: 10-94399 (1998-04-01), None
patent: 2001-294549 (2001-10-01), None
U.S. Appl. No. 10/608,625 to Asako et al., filed Jun. 30, 2003.
U.S. Appl. No. 10/617,034 to Itoh et al., filed Jul. 11, 2003.
Itoh et al., “Chiral alcohol production by NADH-dependent phenylacetaldehyde reductase coupled within situregeneration of NADA,”Eur. J. Biochem. 269, 2002, pp. 2394-2402.
Nakamura et al., “Recent developments in asymmetric reduction of ketones with biocatalysts”,Tetrahedron: Asymmetry Report No. 60, Tetrahedron: asymmetry, 14, (2003), pp. 2659-2681.
Spiliotis et al., “Enhanced Optical Purity of 3-Hydroxyesters Obtained by Baker's Yeast Reduction of 3-Ketoesters”,Tetrahedron Letters, vol. 31, No. 11, 1990, pp. 1615-1616.
Wei et al., “Baker's yeast mediated mono-reduction of 1,3-cyclohexanediones bearing two identical C(2) substituents”,Tetrahedron: Asymmetry, vol. 12, 2001, pp. 229-233.
N. Itoh et al., “Chiral alcohol production by β-ketoester reductase fromPenicillium citrinumcoupled with regeneration system of NADPH”, Journal of Molecular Catalysis B Enzymatic, vol. 22, No. 3-4, Jun. 2, 2003, pp. 247-248.
Itoh et al., “Production of chiral alcohols by enantioselective reduction with NADH-dependent phenylacetaldehyde reductase fromCorynebacteriumstain, ST-10”,Journal of Molecular Catalysis B: Enzymatic, vol. 6, 1999, pp. 41-50.
Itoh et al., “Purification and Characterization of Phenylacetaldehyde Reductase from a Styrene-AssimilatingCorynebacteriumStrain, ST-10”,Applied and Environmental Microbiology, vol. 63, No. 10, Oct. 1997, pp. 3783-3788.
Wang et al., “Cloning, sequence analysis, and expression inEscherichia coliof the gene encoding phenyklacetaldehyde reductase from styrene-assimilatingCorynebacteriumsp. Strain ST-10”,Applied Microbiology Biotechnology, vol. 52, 1999, pp. 386-392.
Itoh et al., “1465. Chiral alcohols production by enantioselective reduction with NADH-dependent phenylacetaldehyde reductase (PAR)”,Book of Abstracts, 2000 International Chemical Congress of Pacific Basin Societies, Dec. 14-19, 2000, p. 9.
Itoh et al., “3Y7p7. Production of optically active alcohol by using a phenylacetaldehyde reductase (PAR) recombinant strain”,Nippon Nogeikagaku Kaishi, vol. 75, Mar. 5, 2001, with translation of 3Y7P7.
Itoh et al., “3F302β. Analysis of the phenylacetaldehyde reductase (PAR) gene from styrene-assimilatingCorynebacterium”, Nippon Nogeikagaku Kaishi, vol. 74, Mar. 5, 2000, with translation of 3F302β.
Itoh et al., “3F303α. Production of optically active alcohol by using the phenylacetaldehyde reductase (PAR) fromCorynebhacteriumsp. ST10”,Nippon Nogeikagaku Kaishi, vol. 74, Mar. 5, 2000, with translation of 3F303α.
Asako et al., “P214. Chiral Alcohol Production by β-Ketoester Reductase fromPenicillum citrinumCoupled with Regeneration System of NADPH”,Chem. Litsy 97, 6th International Symposium on Biocatalysis and Biotransformation, Jun. 28-Jul. 3, 2003, p. 489.
Lecture Summary Series of the 6thOrganism Catalyst Chemistry Symposium, Dec. 12-13, 2002, p. 70, with partial English translation.
Conference Lecture Summary Series, published Mar. 5, 2003, 3A11a01, with partial English translation.
Asako Hiroyuki
Shimizu Masatoshi
Pak Yong D.
Saidha Tekchand
Sughrue & Mion, PLLC
Sumitomo Chemical Company Limited
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