Modified pseudomonas oleovorans phaC1 nucleic acids encoding bis

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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435 691, 4352523, 4352542, 4353201, 435440, 435468, 435471, 536 232, 536 237, 800278, A01H 500, C12N 121, C12N 1531, C12N 1552, C12N 1570, C12N 1581, C12N 1582

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active

061439525

ABSTRACT:
A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.

REFERENCES:
patent: 5229279 (1993-07-01), Peoples et al.
patent: 5245023 (1993-09-01), Peoples et al.
patent: 5250430 (1993-10-01), Peoples et al.
patent: 5371002 (1994-12-01), Dennis et al.
patent: 5395919 (1995-03-01), Lee et al.
patent: 5480794 (1996-01-01), Peoples et al.
patent: 5512456 (1996-04-01), Dennis
patent: 5512669 (1996-04-01), Peoples et al.
patent: 5518907 (1996-05-01), Dennis
patent: 5534432 (1996-07-01), Peoples et al.
patent: 5602321 (1997-02-01), John
patent: 5610041 (1997-03-01), Somerville et al.
patent: 5650555 (1997-07-01), Somerville et al.
patent: 5661026 (1997-08-01), Peoples et al.
patent: 5663063 (1997-09-01), Peoples et al.
Harayama S. "Artificial evolution by DNA shuffling." TIB Tech 16: 76-81, Feb. 1998.
Beevers, "Microbodies in Higher Plants," Annual Review of Plant Physiology, 30, 159-193 (1979).
Brandl et al., "Plastics from Bacteria and for Bacteria: Poly(.beta.-Hydroxy-alkanoates) as Natural, Biocompatible, and Biodegradable Polyesters," Adv. Biochem. Eng. Biotechnol., 41, 77-93 (1990).
Brandl et al., "Pseudomonas oleovorans as a Source of Poly(.beta.-Hydroxyalkanoates) for Potential Applications as Biodegradable Polyesters," Appl. Environ. Microbiol., 54, 1977-1982 (1988).
DiRusso, "Primary Sequence of the Escherichia coli fadBA Operson, Encoding the Fatty Acid-Oxidizing Multienzyme Complex, Indicates a High Degree of Homology to Eucaryotic Enzymes," J. Bacteriol., 172, 6459-6468 (1990).
Eggink et al., "Synthesis of Poly-3-Hydroxy-Alkanoates (PHAs) by Pseudomonas: Substrates Polymerases, Bioreactor Configurations, and Products," Abstract Pap. Am. Chem. Soc., 204 Meeting, Part 2, PMSE73 (1992).
Eschenlauer et al., "Production of a Heteropolymeric Polydroxyalkanoate in Escherichia coli from a single carbon source," Int. J. Biol. Macromol., 19, 121-130 (1996).
Fromm et al., "Expression of genes transferred into monocot and dicot plant cells by electroporation," Proc. Natl. Acad. Sci., USA, 82, 5824-5828 (1985).
Fromm et al., "Stable Transformation of Maize After Gene Transfer by Electroporation," Nature, 319, 791-793 (1986).
Gagnon et al., "Crystallization Behavior and Its Influence on the Mechanical Properties of a Thermoplastic Elastomer Produced by Pseudomonas oleovorans," Macromolecules, 25, 3723-3728 (1992).
Hahn et al., "Growth Kinetics, Nutrient Uptake, and Expression of the Alcaligenes eutrophus Poly(.beta.-hydroxybutyrate) Synthesis Pathway in Transgenic Maize Cell Suspension Cultures," Biotechnol. Prog., 13, 347-354 (1997).
Hahn et al., "Peroxisomal Localization of PHA Synthesis in Eukaryotic Cells," International Symposium on Bacterial Polydydroxyalkanoates '96, (abstract and poster) 16 pgs. (1996).
Haywood et al., "Characterization of Two 3-ketothiolases Possessing Differing Substrate Specificities in the Polyhydroxyalkanoate Synthesizing Organism Alcaligenes eutrophus," FEMS Microbiology Letters, 52, 91-96 (1989).
Haywood et al., "The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus," FEMS Microbiology Letters, 57, 1-6 (1989).
Hernlern et al., "Intracellular PH in Single Sacchromyres cerevisiae Cells," Biotechnol. Techn., 3, 79-84 (1989).
Huang, "Metabolism in Plant Peroxisomes," Recent Advances in Phytochemistry, 16, Ed. Creasey et al., 85-123 (1982).
Huisman et al., "Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans," The Journal of Biological Chemistry, 266, 2191-2198 (1991).
Jackson et al., "Extending the Range of PHA Polymerase Substrate Specificity," (Poster), AIChE Annual Meeting, Session 232 (Nov. 17, 1997, (abstract available no earlier than Mar. 31, 1997)).
Kato et al., "Production of a novel copolyster of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids by Pseudomonas sp. 61-3 from sugars," Appl. Microbiol. Biotech., 45(3), 363-370 (1996).
Kim, "Preparations, Characterization, and Modification of Poly-beta-hydrozyalkanoates from Pseudomonas oleovorans," Ph.D. Thesis, University of Massachusetts, Amherst (1991).
Kim et al., "Poly(.beta.-hydroxyalkanoate) Copolymers Containing Brominated Repeating Units Produced by Pseudomonas oleovorans," Macromolecules, 25, 1852-1857 (1992).
Kindl, "Fatty Acid Degradation in Plant Peroxisomes: Function and Biosynthesis of the Enzymes Involved," Biochemie, 75, 225-230 (1993).
Kitamura et al., "Staining Method of Poly(3-Hydroxyalkanoic Acids) Producing Bacteria by Nile Blue," Biotechnol. Techniq., 8, 345-350 (1994).
Lagaveen et al., "Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates," Appl. Environ. Microbiol., 54, 2924-2932 (1988).
Langenbach et al., "Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate) synthesis," FEMS Microbiol. Lett., 150, 303-309 (1997).
Law et al., "Assay of Poly-.beta.-Hydroxybutyric Acid," J. Bacteriol., 82, 33-36 (1961).
Lazarow et al., "Biogenesis of Peroxisomes," Ann. Rev. Cell Biol., 1, 486-530 (1985).
Leaf et al., "Saccharomyces cerevisiae expressing bacterial polydroxy butyrate synthase produces poly-3-hydroxy butyrate," Microbiology (Reading), 142, 1169-1180 (1996).
Lee, "Bacterial Polyhydroxyalkanoates," Biotechnol. Bioeng., 49, 1-14 (1996).
Lee et al., "Regulatory Effects of Cellular Nicotinamide Nucleotides and Enzyme Activities on Poly(3-Hydroxybutyrate) Synthasis in Recombinant Escherichia coli," Biotechnol. Bioeng., 52, 707-712 (1996).
Lee et al., "Production of poly(3-hydroxybutyric acid) by recombinant Escherichia coli strains: genetic and fermentation studies," Can. J. Microbiol., 41, (suppl. 1), 207-215 (1995).
Lenz et al., "Stereochemistry of the Ring Opening Polymerization of [S]-.beta.-Butyrolactone," Polymer Preprints, 31, 408-409 (1990).
Lorimer et al., "Random recombination of antibody single chain Fv sequences after fragmentation with Dnasel in the presence of Mn.sup.2+," Nuc. Acids Res., 23, 3067-3068 (1995).
Marchessault, "Tender Morsels for Bacteria," Trip, 4, 163-168 (1996).
Ostle et al., "Nile Blue A as a Fluorescent Stain for Poly-.beta.-Hydroxybutyrate," Appl. Environ. Microbiol., 44, 238-241 (1982).
Peoples et al., "Poly-.beta.-hydroxybutyrate (PHB) Biosynthesis in Alcaligenes eutrophus H16," J. Biol. Chem., 264, 15298-15303 (1989).
Ramsay et al., "Effect of nitrogen limitation on long-side-chain poly-beta-hydroxyalkanoate synthesis by Pseudomonas resinovorans," Appl. Environ. Microbiol., 58, 744-746 (1992).
Ren et al., "Substrate specificity of poly-3-hydroxyalkanoate polymerases from Pseudomonas oleovorans GPo1," presented in a poster at the meeting of the International Symposium on Bacterial Polyhydroxyalkanoates 1996, Davos, Switzerland, Aug. 18-23, 1996.
Rhodes et al., "Genetically Transformed Maize Plants from Protoplasts," Science, 240, 204-207 (1988).
Riis et al., "Gas Chromatographic Determination of Poly-.beta.-hyddroxybutyric Acid in Microbial Biomass After Hydrochloric Acid Propanolysis," J. of Chromat., 445, 285-289 (1988).
Sambrook et al., Molecular Cloning: A Laboratory Manual, 2.sup.nd ed., Cold Spring Laboratory Press (1989).
Seidman et al., "High-Efficiency Transformation by Electroporation," Ausubel et al., Eds., Short Protocols in Molecular Biology, John Wiley & Sons, Inc., New York, Third Edition, 1.22-1.23 (1995).
Steinbuchel, "A Pseudomonas strain accumulating polyesters of 3-hydroxybutyric acid and medium-chain-length 3-h

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