Modified proteins comprising controllable intervening protein se

Details Modified proteins comprising controllable intervening protein se Modified proteins comprising controllable intervening protein se

1997-03-05

1998-11-10

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 4351723, 4352523, 4353201, 530350, 530402, 530412, 530413, 536 234, C12N 1562, C07K 1900, C12P 2100, C12P 2104

Patent

active

058342478

ABSTRACT:
The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into or adjacent a target protein, the CIVPS being capable of excision from or cleavage of the modified protein under predetermined conditions in cis or in trans, i.e., increase in temperature, exposure to light, unblocking of amino acid residues by dephosphorylation, treatment with chemical reagents or deglycosylation. If desired, the modified protein can be subjected to these conditions. The CIVPS may also be inserted into a region that substantially inactivates target protein activity. The CIVPS may be used in a number of applications including purification of the target protein in a one-step protocol.

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