Modified prokaryotic glucose isomerase enzymes with altered pH a

Details Modified prokaryotic glucose isomerase enzymes with altered pH a Modified prokaryotic glucose isomerase enzymes with altered pH a

1991-01-04

1994-08-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435 691, 4351723, 4352523, 43525233, 536 232, 935 10, 935 14, 935 60, 935 72, 935 75, C12M 1561, C12M 992, C12M 1570, C12M 1574

Patent

active

053407385

ABSTRACT:
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.

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