Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1995-07-06
1998-09-22
Leary, Louise
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 24, 435212, 435215, 435217, 435218, 435219, 435 4, 435183, 435975, 435970, 435968, 530300, 530333, 530827, C12Q 137, C12Q 100, C12N 966, A61K 3800
Patent
active
058112529
ABSTRACT:
Detection or determination of a protease in a sample by incubating the sample with a substrate of the protease and observing proteolytic cleavage of said substrate. The substrate is a modified proenzyme containing a recognition site, e.g., an activation site, cleavable by said protease. Proteolytic cleavage of the modified proenzyme is detected by observing the resulting activity using a suitable substrate of the activated proenzyme. The protease may be e.g. an aspartic protease or a metalloprotease, and the modified proenzyme e.g. pro-urokinase having a mutant activation site which is cleavable by the protease to be determined.
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Leary Louise
Nederlandse Organisatie voor toegepast-natuurwetenschappelijk On
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