Modified probe molecules having self-complementary regions

Details Modified probe molecules having self-complementary regions Modified probe molecules having self-complementary regions

2007-10-31

2009-08-11

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S024300, C536S024310, C536S024330

Reexamination Certificate

active

07572588

ABSTRACT:
The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

REFERENCES:
patent: 4725536 (1988-02-01), Fritsch et al.
patent: 4766062 (1988-08-01), Diamond et al.
patent: 4797355 (1989-01-01), Stabinsky
patent: 4820630 (1989-04-01), Taub
patent: 4822733 (1989-04-01), Morrison
patent: 4925785 (1990-05-01), Wang et al.
patent: 4996143 (1991-02-01), Heller et al.
patent: 5087558 (1992-02-01), Webster
patent: 5091519 (1992-02-01), Cruickshank
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5403711 (1995-04-01), Walder et al.
patent: 5445933 (1995-08-01), Eadle et al.
patent: 5487972 (1996-01-01), Gelfand et al.
patent: 5514546 (1996-05-01), Kool
patent: 5514551 (1996-05-01), Yang et al.
patent: 5536638 (1996-07-01), Rossau et al.
patent: 5573906 (1996-11-01), Bannwarth et al.
patent: 5574145 (1996-11-01), Barry et al.
patent: 5607834 (1997-03-01), Bagwell
patent: 5641625 (1997-06-01), Ecker et al.
patent: 5645985 (1997-07-01), Froehler et al.
patent: 5652099 (1997-07-01), Conrad
patent: 5656427 (1997-08-01), Hammond et al.
patent: 5679553 (1997-10-01), Van Gemen et al.
patent: 5691146 (1997-11-01), Mayrand
patent: 5723591 (1998-03-01), Livak et al.
patent: 5731148 (1998-03-01), Becker et al.
patent: 5747252 (1998-05-01), Yang et al.
patent: 5808023 (1998-09-01), Sanghvi et al.
patent: 5811533 (1998-09-01), Gold et al.
patent: 5827649 (1998-10-01), Rose et al.
patent: 5837442 (1998-11-01), Tsang et al.
patent: 5846726 (1998-12-01), Nadeau et al.
patent: 5849504 (1998-12-01), Shah et al.
patent: 5866336 (1999-02-01), Nazarenko et al.
patent: 5898031 (1999-04-01), Crooke et al.
patent: 5914396 (1999-06-01), Cook et al.
patent: 5919630 (1999-07-01), Nadeau et al.
patent: 5925517 (1999-07-01), Tyagi et al.
patent: 5928862 (1999-07-01), Morrison
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6020130 (2000-02-01), Gold et al.
patent: 6030787 (2000-02-01), Livak et al.
patent: 6117635 (2000-09-01), Nazarenko et al.
patent: 6130038 (2000-10-01), Becker et al.
patent: 6150097 (2000-11-01), Tyagi et al.
patent: 6156501 (2000-12-01), McGall et al.
patent: 6218107 (2001-04-01), Brentano et al.
patent: 6903206 (2005-06-01), Becker et al.
patent: 7070925 (2006-07-01), Becker et al.
patent: 7399852 (2008-07-01), Becker et al.
patent: 2005/0106610 (2005-05-01), Becker et al.
patent: 2008/0090246 (2008-04-01), Becker et al.
patent: 2008/0090247 (2008-04-01), Becker et al.
patent: 0318245 (1981-05-01), None
patent: 0415901 (1991-03-01), None
patent: 0421725 (1991-04-01), None
patent: 0742287 (1996-11-01), None
patent: 9012116 (1990-10-01), None
patent: 9014442 (1990-11-01), None
patent: 9108480 (1991-06-01), None
patent: 9202258 (1992-02-01), None
patent: 9313121 (1993-07-01), None
patent: 94/01550 (1994-01-01), None
patent: 9402501 (1994-02-01), None
patent: 9415169 (1994-07-01), None
patent: 9419023 (1994-09-01), None
patent: 9514706 (1995-06-01), None
patent: 9522623 (1995-08-01), None
patent: 9532305 (1995-11-01), None
patent: 9606950 (1996-03-01), None
Tyagi et al., “Molecular Beacons: Probes that Fluoresce upon Hybridization”,Nature Biotechnology, vol. 14, p. 303-308, Mar. 1996.
Shimizu et al., “Effects of 5-Methyl Substitution in 2′-O-Methyloligo- (Pyrimidine) Nucleotides on Triple-Helix Formation”, Bioorg Med Chem Lett, 1994; 4(8):1029-32, Elsevier Science Ltd., UK.
Adams et al., “The Biochemistry of the Nucleic Acids,” §7.2.1, 259-260 ( 11th ed. 1992).
Barabino et al., “Targeted snRNP Depletion Reveals an Additional Role for Mammalian U1 snRNP in Spliceosome Assembly,” Cell, 63:293-302 (1990).
Biencowe et al., “Antisense Probing of the Human U4/U6 snRNP with Biotinylated 2′-OMe RNA Oligonucleotides,” Cell, 59:531-539 (1989).
Carmo-Fonseca et al., “In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells,” EMBO, 10(7):1863-1873 (1991).
Carmo-Fonseca et al., “Assembly of snRNP-containing Coiled Bodies Is Regulated in Interphase and Mitosis-Evidence . . . Coiled Body Is a Kinetic Nuclear Structure,” J.Cell.Biol.,120(4):841-852(1993).
Carmo-Fonseca et al., “Transcription-dependent Colocalization of the U1, U2, U4/U6 and U5 snRNPs in Coiled Bodies,” J.Cell.Biol., 117(1):1-14 (1992).
Carmo-Fonseca et al., “Mammalian nuclei contain foci which are highly enriched in compnnents of the pre-mRNA splicing machinery.” EMBO, 10(1):195-206 (1991).
Lamond et al., “Probing the Structure and Function of U2 snRNP with Antisense Oligonucleotides Made of 2′-OMe RNA,” Cell ,58:383-390 (1989).
Palfi et al., “Affinity purification ofTrypanosoma bruceismall nuclear ribonucleoproteins reveals common and specific protein components,” Proc.Natl.Acad.Sci.USA, 88:9097-9101 (1991).
Shibahara et al., “Site-directed cleavage of RNA,” Nucl. Acid. Res., 15(11):4403-4415 (1987).
Sproat et al., “2′-O-Methyloligoribonucleotides:synthesis and applications,” from Eckstein, “Oligonucleotides and Analogues: A Practical Approach,” Chapter 3, pp. 49-86, (1991).
Wang et al., “Relative stabilities of triple helices composed of combinations of DNA, RNA and 2′-O-methyl-RNA . . . ,”Nucl. Acid. Res., 23(7):1157-1164 (1995).
Winnacker, E.L. , “From Genes To Clones,”VCH Verlagsgesellschaft, Weinheim, FRG, 33-34 (1987).
Wolff et al., “Reconsituted mammalian U4/U6 snRNP complements splicing: a mutational analysis,” EMBO, 11(1):345-359 (1992).
Bobst et al., “Effect of the Methylation of the 2′-Hydroxyl Groups in Polyadenylic Acid on its Structure in Weakly Acidic and Neutral Solutions and on its Capability to Form Ordered Complexes with Polyuridylic Acid”,J. Mol. Biol., 46:221-234 (1969).
Burd et al., “Conserved Structures and Diversity of Functions of RNA-Binding Proteins”,Science, 265:615-621 (1994).
Chiang et al., “Antisense Oligonucleotides Inhibit Intercellular Adhesion Molecule 1 Expression by Two Distinct Mechanisms”,J. Biol. Chem., 266(27):18162-18171 (1991).
Corey, “48000-fold Acceleration of Hybridization by Chemically Modified Oligonucleotides”,J. Am. Chem. Soc., 117(36):9373-9374 (1995).
Cummins et al., “Characterization of Fully 2′-modified Oligoribonucleotide Hetero- and Homoduplex Hybridization and Nuclease Sensitivity”,Nucleic Acids Research, 23(11):2019-2024 (1995).
Dean et al., “Inhibition of Protein Kinase C-α Expression in Human A549 Cells by Antisense Oligonucleotides Inhibits Induction of Intercellular Adhesion Molecule 1 (ICAM-1) mRNA by Phorbol Esters”,J. Biol. Chem., 269(23):16416-16426 (1991).
Ecker et al., “Pseudo-Half-Knot Formation with RNA”,Science, 257:958-961 (1992).
Goodchild, “Conjugates of Oligonucleotides and Modified Oligonucleotides: A Review of Their Synthesis and Properties”,Bioconjugate Chemistry, 1(3):165-187 (1990).
Hou et al., “Inhibition of tRNA Aminoacylation by 2′-O-Methyl Oligonucleotides”,Biochemistry, 35(48):15340-15348 (1996).
Inoue et al., “Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H”,FEBS Letter, 215(2):327-330, 1987.
Inoue et al., “Synthesis an

Affiliated with

Also associated with

Rating

Modified probe molecules having self-complementary regions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modified probe molecules having self-complementary regions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified probe molecules having self-complementary regions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4053564