Modified nucleic acid sequences and methods for increasing...

Details Modified nucleic acid sequences and methods for increasing... Modified nucleic acid sequences and methods for increasing...

1998-10-20

2009-12-15

Schnizer, Richard (Department: 1635)

Multicellular living organisms and unmodified parts thereof and

Method of using a transgenic nonhuman animal to manufacture...

The protein is isolated or extracted from milk

C800S013000, C530S350000, C536S023700, C536S024100

Reexamination Certificate

active

07632980

ABSTRACT:
The invention provides modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing the mRNA levels and protein expression of proteins which are known to be, or are likely to be, difficult to express in cell culture systems, mammalian cell culture systems, or in transgenic animals. The preferred “difficult” protein candidates for expression using the recombinant techniques of the invention are those proteins derived from heterologous cells preferably those of lower organisms such as parasites, bacteria, and virus, having DNA coding sequences comprising high overall AT content or AT rich regions and/or mRNA instability motifs and/or rare codons relative to the recombinant expression system to be used.

REFERENCES:
patent: 4873316 (1989-10-01), Meade et al.
patent: 5194587 (1993-03-01), Knapp et al.
patent: 5225534 (1993-07-01), Certa
patent: 5231168 (1993-07-01), Dziegiel et al.
patent: 5304489 (1994-04-01), Rosen
patent: 5395614 (1995-03-01), Knapp et al.
patent: 5530177 (1996-06-01), Bleck et al.
patent: 5543323 (1996-08-01), Ridley et al.
patent: 5643578 (1997-07-01), Robinson et al.
patent: 5646247 (1997-07-01), Barnwell et al.
patent: 5736131 (1998-04-01), Bosch et al.
patent: 5795737 (1998-08-01), Seed et al.
patent: 5856178 (1999-01-01), White et al.
patent: 6114148 (2000-09-01), Seed et al.
patent: 6130062 (2000-10-01), Milland et al.
patent: 6593463 (2003-07-01), Chen et al.
patent: 7354594 (2008-04-01), Chen et al.
patent: 2002/0144299 (2002-10-01), Chen et al.
patent: 2005/0071890 (2005-03-01), Chen et al.
patent: 2005/0235371 (2005-10-01), Chen et al.
patent: 1997 48649 (1998-04-01), None
patent: 727864 (1998-04-01), None
patent: 0264166 (1988-04-01), None
patent: 0359472 (1990-03-01), None
patent: 0741515 (1995-06-01), None
patent: 0 682 115 (1995-11-01), None
patent: 0923308 (1997-11-01), None
patent: WO 91 08216 (1991-06-01), None
patent: WO-91/18922 (1991-12-01), None
patent: WO 94/05796 (1994-03-01), None
patent: WO-94/28930 (1994-12-01), None
patent: WO 95/17085 (1995-06-01), None
patent: WO 96/03051 (1996-02-01), None
patent: WO 97/26911 (1997-07-01), None
patent: WO 97/30158 (1997-08-01), None
patent: WO/ 97/30159 (1997-08-01), None
patent: WO 97/30159 (1997-08-01), None
patent: WO 97 31115 (1997-08-01), None
patent: WO 98 14583 (1998-04-01), None
patent: WO-99/20766 (1999-04-01), None
patent: WO-99/20774 (1999-04-01), None
Product pd(N)6 in the 1995 Pharmacia Biotech catalogue, p. 277, 1995.
Holder et al Nature 317(6034): 270-273, See entire document, especially abstract, and Fig. 2, p. 272, Sep. 1985.
Akashi et al Blood 83(11): 3182-3187, See entire document, especially abstract, and Fig. 1, Jun. 1994.
Wang et al J. Biol. Chem. 264: 21116-21121, See entire document, especially Fig. 2, col. 1, p. 21118, see particularly glycine codons at positions—11 and 18, Dec. 1989.
Liebhaber, Nucleic Acids Symp Ser, 1997; (36): 29-32.
Lukashov et.al., 1996; Accession L48364.
Simons et al (Bio/Technology 6: 179-183, 1988).
Gordon et al.; Genetic Transformation of Mouse Enbryos by Microinjection of Purified DNA; (1980);Proc. Natl. Acad. Sci.; 77: pp. 7380-7384.
Gordon et al.; Integration and Stable Germ Line Transmission of Genes Injected into Mouse Pronuclei; (1981);Science; 214: pp, 1244-1246.
Brinster et al.; Factors Affecting the Efficiency of Introducing Foreing DNA into Mice by Microinjecting Eggs; (1985);Proc. Natl. Acad. Sci.; 82: pp. 4438-4442.
Palmiter et al.; Transgenic Mice; (1985);Cell; 41: pp. 343-345.
Wall et al.; Development of Porcine Ova That Were Centrifuged to Permit Visualization of Pronuclei and Nuclei1; (1985);Biol. Reprod.; 32: pp. 645-651.
Shaw et al.; A Conserved AU Sequence from the 3′ Untranslated Region of GM-CSF mRNA Mediates Selective mRNA Degradation; (1986);Cell; 46: pp. 659-667.
Chang et al.; Generalized Immunological Recognition of the Major Merozoite Surface Antigen (gp195) ofPlasmodium falciparum; (1989);Proc. Natl. Acad. Sci.; 86: pp. 6343-6347.
Vilotte et al.; Efficient Tissue-Specific Expression of Bovine αlactalbumin in Transgenic Mice; (1989);Eur. J. Biochem.; 186 pp. 43-48.
Buhler et al.; Rabbit β-Casein Promotor Directs Secretion of Human Interleukin-2 into the Milk of Transgenic Rabbits; (1990);Bio/Technology; 8: pp. 140-143.
Ebert et al.; Transgenic Production of A Variant of Human Tissue-Type Plasminogen Activator in Goat Milk Generation of Transgenic Goats . . . ; (1991);Bio/Technology; 9: pp. 835-838.
Krimenfort et al.; Generation of Transgenic Dairy Cattle Using ‘In Vitro’ Embryo Production; (1991)Bio/Technology; 9: pp. 844-847.
Wright et al.; High Level Expression of Active Human Alpha-1-Antitrypsin in the Milk of Transgenic Sheep; (1991);Bio/Technology; 9: 830-834.
Chang et al.; A Carboxyl-Terminal Fragment ofPlasmodium falciparumgp195 Expression by a Recombinant Baculovirus Induces Antibodies that . . . ; (1992);J. Immunol; 149: pp. 548-555.
Soulier et al.; Expression Analysis of Ruminant α-Lactalbumin in Transgenic Mice: Developmental Regulation and General Location of ImportantCis-Regulatory . . . ; (1992);FEBS Letters: 297(1-2): pp. 13-18.
Wall et al.; Making Transgenic Livestock: Genetic Engineering on a Large Scale: (1992);J. Cell. Biochem.; 49: pp. 113-120.
Diggs et al.; The Major Merozoite Surface Protein as a Malaria Vaccine Target; (1993);Parasitology Today; 9(8): pp. 300-302.
Campbell et al.; Sheep Coloned by Nuclear Transfer from a Cultured Cell Line; (1996);Nature; 380: pp. 64-66.
Chang et al.; A Recombinant Baculovirus 42-Kilodalton C-Terminal Fragment ofPlasmodium falciparumMerozoite Surface Protein 1 ProtectsAotusMonkeys . . . ; (1996);Infection&Immunity; 64(1): pp. 253-262.
Hochi et al.; Secretion of Bovine α-Lactalbumin into the Milk of Transgenic Rats, (1992)Molecular Reproduction and Development33:160-164.
Chattergoon et al., Genetic immunization: a new era in vaccines and immune therapeutics. FASEB J. Aug. 1997:11(10);753-63.
Dame et al., Current status of thePlasmodium falciparumgenome project. Mol Biochem Parasitol. Jul. 1996;79(1):1-12.
D'Orso et al., TcUBP-1, a developmentally regulated U-rich RNA-binding protein involved in selective mRNA destabilization in trypanosomes. J Biol Chem. Sep. 14, 2001;276(37):34801-9 Epub Jul. 2, 2001.
Gardner et al., DNA vaccines against malaria: immunogenicity and protection in a rodent model. J Pharm Sci. Dec. 1996;85(12):1294-300.
Graves et al., Comparison of the cost-effectiveness of vaccines and insecticide impregnation of mosquito nets for the prevention of malaria. Ann Trop Med Parasitol. Jun. 1998;92(4):399-410.
Graves et al., Vaccines for preventing malaria. Cochrane Database Syst Rev. 2003;(1):CD000129.
Gutierrez et al., Expression of a bovine kappa-CN cDNA in the mammary gland of transgenic mice utilizing a genomic milk protein gene as an expression cassette, Transgenic Res. Jul. 1996;5(4):271-9.
Jenkins et al., Evolution of base composiiton and codon usage bias in the genus Flavivirus. J Mol Evol. Apr. 2001;52(4):383-90.
Jongwutiwes et al., Sequence conservation in the C-terminal part of the precursor to the major merozoite surface proteins (MSP1) ofPlasmodium falciparumfrom field isolates. Mol Biochem Parasitol. May 1993;59(1):95-100.
Kalinna et al., DNA vaccines for parasitic infections. Immunol Cell Biol. Aug. 1997;75(4):370-5.
Ledley et al., Clinical considerations in the design of protocols for somatic gene therapy. Hum Gene Ther. 1991 Spring;2(1):77-83.
Martin et al., Total synthesis and expression inEscherichia coliof a gene encoding human tropoelastin. Gene. Mar. 10, 1995;154(2):159-66.
McDonnell et al., DNA vaccines. N Engl J Med. Jan. 4, 1996;334(1):42-5.
Nuijens et al., Characterization of recombinant human lactoferrin secreted in milk of transgenic mice. J Biol Chem. Mar. 28, 1997;272(13):8802-7.
Orkin et al., Report and recommendations of panel to assess NIH investment in Gene Therapy Res. 1995.
Perlak et al., Modification of the coding sequence enhances plant expression of insect control protein genes. Proc Natl Acad Sci U S A. Apr. 15, 1991;8

Affiliated with

Also associated with

Rating

Modified nucleic acid sequences and methods for increasing... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modified nucleic acid sequences and methods for increasing..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified nucleic acid sequences and methods for increasing... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4130072