Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2011-07-26
2011-07-26
Fronda, Christian L (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S183000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07985572
ABSTRACT:
The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-typeAlcaligenes faecaliscomprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.
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English Translation of Notice of Reasons for Rejection Dated Jan. 5, 2010 Issued in Japanese Application No. 2006-501937.
Friedrich Thomas
Hauer Bernhard
Keβeler Maria
Zelinski Thomas
BASF SE
Connolly Bove & Lodge & Hutz LLP
Fronda Christian L
LandOfFree
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