Modified N-4 nucleotides for use in amplified nucleic acid hybri

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 243, 435 5, 435 6, 435 911, C07H 2102, C07H 2104, C12Q 168, C12Q 170

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055941184

ABSTRACT:
Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.

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