Modified maxadilan protein, its preparation and use, and DNA enc

Details Modified maxadilan protein, its preparation and use, and DNA enc Modified maxadilan protein, its preparation and use, and DNA enc

1993-08-06

1996-01-02

Wax, Robert A.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

435 691, 435 697, 435214, 435217, 514 12, 530300, 530324, 536 221, 536 231, 536 232, 536 234, 536 235, 536 241, A61K 3800, C12P 2106, C12N 948, C07H 1900

Patent

active

054808642

ABSTRACT:
A modified maxadilan protein exhibits higher biological activity than native maxadilan from the sand fly Lutzomyia longipalpis. A modified maxadilan fusion protein contains a thrombin cleavage site. This enables the production of the modified maxadilan as a fusion protein and recovery of the modified maxadilan after digestion with thrombin. The modified maxadilan is a potent vasodilator.

REFERENCES:
International Search Report PCT/US94/08809 Lerner et al., Ethan A., "Maxadilan Cloning and Functional Expression of the Gene Encoding This Potent Vasodilator Peptide," J. Bio. Chem., vol. 267, No. 2, pp. 1062-1066 (1992).
Mandel, M., et al., Calcium-dependent Bacteriophage DNA Infection, J. Mol. Biol., 53:159-162 (1970).
Lerner, E., et al., Maxadilan--Cloning and Functional Expression of the Gene Encoding This Potent Vasodilator Peptide, Journal of Biological Chemistry, vol. 267, No. 2, Issue of Jan. 15, 1992, pp. 1062-1066.
Smith D., et al., Single-step Purification of Polypeptides Expressed in Escherichia coli as Fusions with Glutathione S-transferase, Gene, vol. 67, No. 1, Issue of Jul. 15, 1988, pp. 31-40.
Ribeiro et al., A Novel Vasodilatory Peptide from the Salivary Glands of the Sand Fly Lutzomyia longipalpis, Science, vol. 243, pp. 212-214 (1989).
Chang, Thrombin specificity--Requirement for Apolar Amino Acids Adjacent to the Thrombin Cleavage Site of Polypeptide Substrate, Eur. J. Biochem., vol. 151, pp. 217-224 (1985).

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