Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2007-02-27
2007-02-27
Slobodyansky, Elizabeth (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S252300, C435S320100, C435S325000, C536S023200
Reexamination Certificate
active
10954840
ABSTRACT:
The invention comprises modified luciferase proteins which are more resistant to inhibition by test chemicals than wild type luciferase. The modified luciferases also contain greater thermostability than wild type luciferase.
REFERENCES:
patent: 5219737 (1993-06-01), Kajiyama et al.
patent: 5229285 (1993-07-01), Kajiyama et al.
patent: 5650289 (1997-07-01), Wood
patent: 5670356 (1997-09-01), Sherf et al.
patent: 6265177 (2001-07-01), Squirrell et al.
Andreotti, et al. (1994) Bioluminescence and Chemiluminescence: Fundamentals and Applied Aspects, Proceedings of the International Symposium on Bioluminescence and Chemiluminescence, 8th, Cambridge, UK, Sep. 5-8, 1994, pp. 403-406. Editor(s): Campbell et al., Publisher: Wiley, Chichester, UK.
Kim-Choi et al (2006) Luminescence, vol. 21, No. 3, pp. 135-142.
Kim-Choi et al (2006) Toxicology in Vitro, vol. 20, pp. 1537-1547.
Andreotti et al., “Sensitivity Testing of Human Tumors Using a Microplate ATP Assay,”Bioluminescence and Chemiluminescence, (Campbell, Kricka, Stanley eds.) pp. 403-406.
Branchini et al., “Identification of a Firefly Luciferase Active Site Peptide Using a Benzophenone-based Photooxidation Reagent,”J. Biological Chemistry, (1997) 272-31:19359-19364.
Branchini et al., “Site-Directed Mutagenesis of Histidine 245 in Firefly Luciferase: A Proposed Model of the Active Site,”Biochemistry, (1998) 37:15311-15319.
Branchini et al., “Site-Directed Mutagenesis of Firefly Luciferase Active Site Amino Acids: A Proposed Model for Bioluminescence Color,”Biochemistry, (1999) 38:13223-13230.
Branchini et al., “The Role of Active Site Residue Arginine 218 in Firefly Luciferase Bioluminescence,”Biochemistry, (2001) 40:2410-2418.
Conti et al., “Crystal Structure of Firefly Luciferase Throws Light on a Superfamily of Adenylate-Forming Enzymes,”Structure, (1996) 4:287-298.
DeLuca et al., “Purification and Properties of Firefly Luciferase,”Methods in Enzymology, (1978) 57:3-15.
DeWet et al., “Cloning of Firefly Luciferase cDNA and the Expression of Active Luciferase inEscherichia coli,” Proc. Natl. Acad. Sci., (1985) 82:7870-7873.
DeWet et al., “Firefly Luciferase Gene: Structure and Expression in Mammalian Cells,”Molecular and Cellular Biology, (1987) 7:2:725-737.
Kajiuama et al., “Isolation and Characterization of Mutants of Firefly Luciferase Which Produce Different Colors of Light,”Protein Engineering, (1991) 4:6:691-693.
Kajiyama et al., “Thermostabilization of Firefly Luciferase by a Single Amino Acid Substitution,”Biochemistry, (1993) 32:13795-13799.
Kim et al., “Creating Mutant Luciferase Resistant to HPV Chemical Inhibition by Random Mutagenesis and Colony Level Screening,” Abstract.
Kim et al., “Characterization of Chloroform Resistant Mutant Luciferase, CNBluc03-06,” Abstract.
Kricka L.J., “Chemiluminescence and Bioluminescence,”Analytical Chemistry, (1995) 67:12:499R-502R.
Lundin A., “Use of Firefly Luciferase in ATP-Related Assays of Biomass, Enzymes, and Metabolites,”Methods in Enzymologyl, (2000) 305:346-370.
Price et al., “Luciferase and Recombinant Luciferase Labels,”J. Clinical Ligand Assay, (1998) 21:4:349-357.
Seliger et al., “Spectral Emission and Quantum Yield of Firefly Bioluminescence,”Archives of Biochemistry and BiophysicsI, (1960) 33:136-141.
Simpson et al., “The Effect of Detergents of Firefly Luciferase Reactions,”J. Bioluminescence and Chemiluminescence, (1991) 6:97-106.
Tisi et al., “Development of a Thermostable Firefly Luciferase,”Analytica Chimica Acta, (2002) 457:115-123.
Thompson et al., “Mutation of a Protease-sensitive Region in Firefly Luciferase Alters Light Emission Properties,”J. Biological Chemistry, (1997) 272:30:18766-18771.
Wakuri et al., “Cytotoxicity Study of 32 Meic Chemicals by Colony Formation and ATP Assays,”Toxic. In Vitro, (1993) 7:4:517-521.
White et al., “Improved Thermostability of the North American Firefly Luciferase: Saturation Mutagenesis at Position 354,”Biochem J., (1996) 319:343-350.
Wood et al., “Introduction of Beetle Luciferases and their Applications,”J. Bioluminescence and Chemiluminescence, (1989) 4:289-301.
Gould et al., “Firefly Luciferase as a Tool in Molecular and Cell Biology,”Analytical Biochemistry, (1988)175:5-13.
Lundin, Arne, “Applications of Firefly Luminescence,”Bioluminescence and Chemiluminescence, (1981) 187-197.
Baldwin, T.O., “Firefly luciferase: the structure is known, but the mystery remains,” Structure (1996) 4:223-228.
Choi Eileen H. Kim
Rybina Irina V.
Shonnard David R.
Board of Control of Michigan Technological University
Michael & Best & Friedrich LLP
Peterson Jeffrey D.
LandOfFree
Modified luciferase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Modified luciferase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified luciferase will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3894565