Modified lepidopteran receptors and hybrid multifunctional...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S007200, C435S069100, C435S072000, C435S173300, C435S252300, C435S320100, C435S410000, C536S023100, C536S023400, C536S023500, C530S300000, C530S350000

Reexamination Certificate

active

06875569

ABSTRACT:
In accordance with the present invention, it has been discovered that nuclear receptor proteins isolated from the silk mothbombyx mori(bR) are useful for the regulation of transgene expression. bR is generally thought to be a strong transcriptional regulator within cells of the silk moth. In accordance with the present invention, it has been discovered that bR is also functional in mammalian cells. It has further been discovered that the addition of activation domains to the bR open-reading frame enhances the activity of the ligand modulated regulator to afford high-level transcriptional induction. Further modifications to the bR ligand binding domain result in receptors with unique transactivational characteristics.

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