Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-12-18
1999-02-09
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435184, 435219, 435 23, 435 41, 530351, 536 235, C12N 964, C12N 999, C07K 100, C07H 2104
Patent
active
058693157
ABSTRACT:
Modified forms of human interleukin-1.beta. converting enzyme (ICE) that display proteolytic activity and, furthermore, have increased stability compared to unmodified human ICE are disclosed. Nucleic acid molecules encoding a modified p10 subunit of ICE, and recombinant vectors and host cells incorporating such nucleic acid molecules, are also disclosed. A modified ICE protein of the invention can be used to cleave proteolytically ICE substrates and to identify modulators of ICE activity in screening assays. Moreover, due to its enhanced stability, the modified ICE of the invention is particularly suitable for use in the preparation of ICE crystals for X-ray crystallography.
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Ferenz Catherine R.
Ghayur Tariq
Mankovich John A.
Talanian Robert V.
BASF - Aktiengesellschaft
DeConti, Jr. Guilio A.
Kara Catherine J.
Saidha Tekchand
Wax Robert A.
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