Modified green fluorescent proteins

Details Modified green fluorescent proteins Modified green fluorescent proteins

1996-11-20

1998-07-07

Wax, Robert A..

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530855, 435 691, 435 697, 4351721, 435189, C07K 100, C12P 2104, C12N 1500, C12N 902

Patent

active

057770794

ABSTRACT:
Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

REFERENCES:
patent: 5264563 (1993-11-01), Huse
patent: 5491084 (1996-02-01), Chalfie
patent: 5625048 (1997-04-01), Tsien et al.
Heim et al., "Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy tranfer," Current Biology 6(2): 178-182 (1996).
Inouye and Tsuji, "Expression of the gene and fluorescence characteristics of the recombinant protein," FEBS Letters 341: 277-280 (1994).
Mitra et al., "Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein," Gene 173(1): 13-17 (Jul 1, 1996).
Roth, Thesis from the Graduate Program in Biochemistry from Rutgers, The State University of New Jersey (Oct. 1985).

Affiliated with

Also associated with

Rating

Modified green fluorescent proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modified green fluorescent proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified green fluorescent proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1207223