Modified gene-silencing RNA and uses thereof

Details Modified gene-silencing RNA and uses thereof Modified gene-silencing RNA and uses thereof

2003-03-12

2009-08-18

Mehta, Ashwin (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide encodes an inhibitory rna molecule

C435S419000, C435S468000, C435S320100

Reexamination Certificate

active

07576262

ABSTRACT:
Methods and means for efficiently downregulating the expression of any gene of interest in eukaryotic cells and organisms are provided. To this end, the invention provides modified antisense and sense RNA molecules, chimeric genes encoding such modified antisense or sense RNA molecules and eukaryotic organisms such as plants, animals or fungi, yeast or molds, comprising the modified antisense and/or sense RNA molecules or the encoding chimeric genes.

REFERENCES:
patent: 4873191 (1989-10-01), Wagner et al.
patent: 5037745 (1991-08-01), McAllister
patent: 5190931 (1993-03-01), Inouye
patent: 5908779 (1999-06-01), Carmichael et al.
patent: 0 223 399 (1987-05-01), None
patent: 0 240 208 (1987-10-01), None
patent: 0 467 349 (1992-01-01), None
patent: WO 89/03887 (1989-05-01), None
patent: WO 89/10396 (1989-11-01), None
patent: WO 92/13956 (1992-08-01), None
patent: WO 95/15394 (1995-06-01), None
patent: WO 96/06932 (1996-03-01), None
patent: WO 97/13865 (1997-04-01), None
patent: WO 98/05770 (1998-02-01), None
patent: WO 01/12824 (2001-02-01), None
patent: WO 02/00894 (2002-01-01), None
patent: WO 02/00904 (2002-01-01), None
patent: WO 02/10365 (2002-02-01), None
Thomas et al., Plant Journal, 2001, vol. 25, pp. 417-425.
J.M. Alonso et al., “EIN2, a Bifunctional Transducer of Ethylene and Stress Responses inArabidopsis”, Science, Jun. 25, 1999, vol. 284, p. 2148-2152, American Association for the Advancement of Science with the assistance of Stanford University Libraries' HighWire Press, Stanford, California, USA.
Yong-Qiang An et al, “Conserved Expression of theArabidopsis ACT1andACT3Actin Subclass in Organ Primordia and Mature Pollen”,The Plant Cell, vol. 8, p. 15-30, Jan. 1996, American Society of Plant Physiologists, Rockville, Maryland USA.
F. Bussiere et al., “Compilation and Analysis of Viroid and Viroid-Like RNA Sequences”,Nucleic Acids Research, 1996, vol. 24, No. 10, p. 1793-1798, Oxford University Press, Oxford, United Kingdom.
E. Butler et al, “Bacteriophage SP6-specific RNA Polymerase”,The Journal of Biological Chemistry, vol. 257, No. 10, Issue of May 25, p. 5772-5778, American Society for Biochemistry and Molecular Biology, Bethesda, MD, USA.
R.R.D. Croy, Plant Molecular Biology Labfax: 1993, Blackwell Scientific Publications, UK, p. 180-182.
M. Dalrymple et al., “Genetically Modified Livestock for the Production of Human Proteins in Milk”,Biotechnol. Genet. Eng. Rev., vol. 15, p. 33-49, Apr. 1998, Intercept, Newcastle upon Tyne, UK.
B. Davis et al, “Expansion of CUG Trinucleotide Repeat in the 3′ Untranslated Region of Myotonic Dystrophy Protein Kinase Transcripts Results in Nuclear Retention of Transcripts”,Proc. Natl. Acad. Sci. USA, vol. 94, p. 7388-7393, National Academy of Sciences, USA.
J. Dunn et al., “Complete Nucleotide Sequence of Bacteriophase T7 DNA and the Locations of T7 Genetic Elements”,J. Mol. Biol., 1983, vol. 166, p. 477-535, Academic Press Inc., San Diego, CA USA.
J. Dunn et al, “Different Template Specificities of Phase T3 and T7 RNA Polymerases”,Nature New Biology, vol. 230, Mar. 17, 1971, MacMillan Journals Limited, London England, p. 94-96.
C. Fagoaga et al, “A Citrus Exocortis Viroid Variant from Broad Bean (Vicia fabaL.): Infectivity and Pathogenesis”,Journal of General Virology, 1995, vol. 76, p. 2271-2277, Society for General Microbiology, London, UK.
A. Gleave, “A Versatile Binary Vector System with a T-DNA Organisational Structure Conductive to Efficient Integration of Cloned DNA Into the Plant Genome”,Plant Molecular Biology, vol. 20, p. 1203-1207, 1992, Kluwer Academic Publisher,Dordrecht, The Netherlands.
M. Harpster et al, “Relative Strengths of the 35S Califlower Mosaic Virus, 1′, 2′, and Nopaline Synthase Promoters in Transformed Tobacco Sugarbeet and Oilseed Rape Callus Tissue”,Mol Gen Genet, 1988, vol. 212, p. 181-190, Springer Verlag, Berlin, Germany.
J. Haseloff et al, “Viroid RNAs of Cadang-Cadang Disease of Coconuts”,Naturevol. 299, p. 316-321, Nature Publishing Group, Hampshire, UK, 1982.
R. Hausmann, “Bacteriophase T7 Genetics” (book),Current Topics in Microb, and Imm., 1976, p. 77-109, Berlin Springer Verlag, New York.
T. Herold et al, “Sequence Analysis of Five New Field Isolates Demonstrates that the Chain Length of Potato Spindle Tuber Viroid (PSTVd) is Not Strictly Conserved But as Variable as in Other Viroids”,Plant Molecular Biology, vol. 19, p. 329-333, 1992,Kluwer Academic Publishers, Dordrecht, The Netherlands.
Yau-Heiu Hsu et al, “Nucleotide Sequence of a Hop Stunt Viroid Variant Isolated from Citrus Growing in Taiwan”,Virus Genes, 1994, vol. 9, No. 2, p. 193-195, Kluwer Academic Publishers, Boston, Manufactured in The Netherlands.
R. Hudspeth et al, “Structure and Expression of the Maize Gene Encoding the Phosphoenolpyruvate Caboxylase Isozyme Involved in C4 Photosynthesis”,Plant Molecular Biology, 1989, vol. 12, p. 579-589, Kluwer Academic Publishers, Dordrecht, The Netherlands.
M. Keil et al, “Both Wound-Inducible and Tuber-Specific Expression are Mediated by the Promoter of a Single Member of the Potato Proteinase Inhibitor II Gene Family”,The EMBO Journal, 1989, vol. 8, No. 5, p. 1323-1330, Oxford University Press, Oxford UK.
B. Keller et al, “Glycine-Rich Cell Wall Proteins in Bean: Gene Structure and Association of the Protein with the Vascular System”,The EMBO Journal, vol. 7, No. 12, p. 3625-2633, 1988, IRL Press Limited, Oxford, England.
B. Keller et al, “Specific Expression of a Novel Cell Wall Hydroxyproline-Rich Glycoprotein Gene in Lateral Root Initiation”,Genes&Developmentvol. 3, p. 1639-1646, 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY USA.
J. Klement et al., “Discrimination Between Bacteriophage T3 and T7 Promoters by the T3 and T7 RNA Polymerases Depends Primarily Upon a Three Base-pair Region Located 10 to 12 Base-pairs Upstream from the Start Site”,J. Mol. Biol., 1990, vol. 215, p. 21-29, Academic Press Limited, San Diego, CA, USA.
K. Koch et al, “Giant Hairpins Formed by CUG Repeats in Myotonic Dystrophy Messenger RNAs Might Sterically Block RNA Export Through Nuclear Pores”,J. Theor. Biol., 1998, vol. 192, p. 505-514, Academic Press, San Diego, CA USA.
K. Korsten et al, “The Strategy of Infection as a Criterion for Phylogenetic Relationships of Non-Coli Phages Morphologically Similar to Phage T7”,J. Gen. Virol, 1979, vol. 43, p. 57-73, Society for General Microbiology, London, UK.
Lee et al, “Nucleotide Sequence of the Korean Strain of Hop Stunt Viroid (HSV)”,Nucleic Acids Research, vol. 16, No. 17, 1988, IRL Press Limited, Oxford, England, p. 8708.
N.J. McGraw et al, “Sequence and Analysis of the Gene for Bacteriophage T3 RNA Polymerase”,Nucleic Acids Research, vol. 13, No. 18, 1985, IRL Press Limited, Oxford, England, p. 6753-6766.
J. Peleman et al, “Structure and Expression Analyses of the S-Adenosylmethionine synthetase Gene Family inArabidopsis thaliana”, Gene, vol. 84, 1989, p. 359-369, Elsevier Science Publishers B.V., Oxford, UK.
M. Perry et al., “Transgenesis in Chickens”,Transgenic Research, vol. 2, 1993, p. 125-133, Kluwer Academic Publishers, Dordrecht, The Netherlands.
N. Rudolph et al, “Biopharmaceutical Production in Transgenic Livestock”,TIBTECH, 1999, vol. 17, p. 367-374, Elsevier Science Ltd. , Netherlands.
B. Tian et al, “Expanded CUG Repeat RNAs Form Hairpins That Activate the Double-Stranded RNA-Dependent Protein Kinase PKR”,RNA, 2000, vol. 6, p. 79-87, Cambridge University Press, Printed in the USA.
H. Towle, “Purification and Characterization of Bacteriophage gh-1-induced Deoxyribonucleic Acid-dependent Ribonucleic Acid Polymerase fromPseudomonas putida”, The Journal of Biological Chemistry, vol. 250, No. 5, Issue of Mar.

Affiliated with

Also associated with

Rating

Modified gene-silencing RNA and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modified gene-silencing RNA and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified gene-silencing RNA and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4063462