Modified freeze fracture direct imaging apparatus and technique

Details Modified freeze fracture direct imaging apparatus and technique Modified freeze fracture direct imaging apparatus and technique

2006-01-24

2010-10-19

Jarrett, Lore (Department: 1797)

Chemistry: analytical and immunological testing

Process or composition for determination of physical state...

Surface area, porosity, imperfection, or alteration

Reexamination Certificate

active

07816141

ABSTRACT:
A modified freeze direct imaging of a viscous surfactant mesophase method. A chamber is provided having controlled temperature and solvent partial pressure. The chamber has two copper planchettes at the top and bottom thereof. A sample is placed in the chamber on a grid and is squeezed between the planchettes into a thin film. The thin film is placed in a liquid to vitrify the sample. The sample is removed from the planchettes to fracture the sample. The sample is then placed on a cold stage; and imaged.

REFERENCES:
patent: 2002/0001855 (2002-01-01), Prentiss et al.
Semmler et al., High-pressure freezing causes structural alterations in phospholipid model membranes, 1998, Journal of Microscopy, v. 190, p. 317-327.
Lepault et al., Electron microscopy of frozen biological suspensions, 1983, Journal of Microscopy, v. 129, p. 89-102.

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