Modified fluorescent proteins and methods for using same

Details Modified fluorescent proteins and methods for using same Modified fluorescent proteins and methods for using same

2011-07-05

2011-07-05

Monshipouri, Maryam (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S069100, C435S320100

Reexamination Certificate

active

07972834

ABSTRACT:
Embodiments of the present invention provide nucleic acid molecules encoding improved fluorescent mutants of the Katushka fluorescent protein, variants and derivatives thereof, as well as proteins and peptides encoded by these nucleic acids. Also of interest are proteins that are substantially similar to, or derivatives, or homologues, or mutants of, the above-referenced specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies specific to the proteins and peptides of the invention. In addition, host-cells, stable cell lines and transgenic organisms comprising the above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells or cell organelles. Finally, kits for use in such methods and applications are provided.

REFERENCES:
Shcherbo et al., Nature Methods, 4(9), 741-745, 2007.

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