Modified flavin adenine dinucleotide dependent glucose...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C435S200000, C435S014000, C435S069100, C435S091100, C435S320100, C435S252300, C435S325000, C536S023200, C536S023100

Reexamination Certificate

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07662600

ABSTRACT:
An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably anAspergillusfungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

REFERENCES:
patent: 2003/0232418 (2003-12-01), Takeshima et al.
patent: 2006/0063217 (2006-03-01), Omura et al.
patent: 1367120 (2003-03-01), None
patent: 1 862 543 (2007-12-01), None
patent: 2004-344145 (2004-12-01), None
patent: WO 2004/058958 (2004-07-01), None
patent: WO 2006/101239 (2006-09-01), None
Bak et al.,Biochimica et Biophysica Acta, 139(2): 277-293 (1967).

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