Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-21
2000-10-03
Kunz, Gary L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 4352523, 435325, 4353201, 530350, 536 234, 536 235, C12N 1512, C12N 1563, C12N 500, C07K 14435
Patent
active
061271497
ABSTRACT:
Disclosed herein are a modified epimorphin obtained by adding a hydrophilic peptide composed of 5 to 99 amino acids to at least one terminus of a polypeptide containing the functional domain of epimorphin, and a modified epimorphin composed of a polypeptide having a structure wherein a hydrophobic domain adjacent to the C-terminus of the whole-length epimorphin consisting of a coiled coil domain (1) on the N-terminal side, a functional domain (2) at the center, a coiled coil domain (3) on the C-terminal side and the hydrophobic domain (4) adjacent to the C-terminus has been deleted from the whole-length epimorphin, and at least part of amino acids have been deleted from the terminal side of at least one of the coiled coil domains (1) and (3) as well. The invention also discloses a variant modified epimorphin obtained by making partial substitution, deletion and/or insertion of amino acids in the amino acid sequence of the modified epimorphin, wherein the variant maintains the function of the original sequence. The invention further discloses DNAs encoding the modified epimorphin and variant thereof, recombinant vectors containing the DNAs, transformants obtained by introducing the recombinant vectors, and a production method of the modified epimorphin and the variants thereof making use of the transformant.
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Hirai Yohei
Koshida Shogo
Oka Yumiko
Kunz Gary L.
Saoud Christine
Sumitomo Electric Industries Ltd.
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