Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2000-11-02
2003-09-23
Achutamurthy, Ponnathapu (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S069100, C435S221000, C435S252300, C435S320100, C435S471000, C536S023200, C510S320000
Reexamination Certificate
active
06623950
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to polypeptide-polymer conjugates having added and/or removed one or more attachment groups for coupling polymeric molecules on the surface of the 3D structure of the polypeptide, a method for preparing polypeptide-polymer conjugates of the invention, the use of said conjugated for reducing the immunogenicity and allergenicity, and compositions comprising said conjugate.
BACKGROUND OF THE INVENTION
The use of polypeptides, including enzymes, in the circulatory system to obtain a particular physiological effect is well-known in the medical arts. Further, within the arts of industrial applications, such as laundry washing, textile bleaching, person care, contact lens cleaning, food and feed preparation enzymes are used as a functional ingredient. One of the important differences between pharmaceutical and industrial application is that for the latter type of applications (i.e. industrial applications) the polypeptides (often enzymes) are not intended to enter into the circulatory system of the body.
Certain polypeptides and enzymes have an unsatisfactory stability and may under certain circumstances—dependent on the way of challenge—cause an immune response, typically an IgG and/or IgE response.
It is today generally recognized that the stability of polypeptides is improved and the immune response is reduced when polypeptides, such as enzymes, are coupled to polymeric molecules. It is believed that the reduced immune response is a result of the shielding of (the) epitope(s) on the surface of the polypeptide responsible for the immune response leading to antibody formation by the coupled polymeric molecules.
Techniques for conjugating polymeric molecules to polypeptides are well-known in the art.
One of the first commercially suitable techniques was described back in the early 1970's and disclosed in e.g. U.S. Pat. No. 4,179,337. Said patent concerns non-immunogenic polypeptides, such as enzymes and peptide hormones coupled to polyethylene glycol (PEG) or polypropylene glycol (PPG). At least 15% of polypeptides' physiological activity is maintained.
GB patent no. 1,183,257 (Crook et al.) describes chemistry for conjugation of enzymes to polysaccharides via a triazine ring.
Further, techniques for maintaining of the enzymatic activity of enzyme-polymer conjugates are also known in the art.
WO 93/15189 (Veronese et al.) concerns a method for maintaining the activity in polyethylene glycol-modified proteolytic enzymes by linking the proteolytic enzyme to a macromolecularized inhibitor. The conjugates are intended for medical applications.
It has been found that the attachment of polymeric molecules to a polypeptide often has the effect of reducing the activity of the polypeptide by interfering with the interaction between the polypeptide and its substrate. EP 183 503 (Beecham Group PLC) discloses a development of the above concept by providing conjugates comprising pharmaceutically useful proteins linked to at least one water-soluble polymer by means of a reversible linking group.
EP 471,125 (Kanebo) discloses skin care products comprising a parent protease (Bacillus protease with the trade name Esperase®) coupled to polysaccharides through a triazine ring to improve the thermal and preservation stability. The coupling technique used is also described in the above mentioned GB patent no. 1,183,257 (Crook et al.).
JP 3083908 describes a skin cosmetic material which contains a transglutaminase from guinea pig liver modified with one or more water-soluble, substances such as PEG, starch, cellulose etc. The modification is performed by activating the polymeric molecules and coupling them to the enzyme. The composition is stated to be mild to the skin.
However, it is not always possible to readily couple polymeric molecules to polypeptides and enzymes. Further, there is still a need for polypeptide-polymer conjugates with an even more reduced immunogenicity and/or allergenicity.
SUMMARY OF THE INVENTION
It is the object of the present invention to provide improved polypeptide-polymer conjugates suitable for industrial and pharmaceutical applications.
The term “improved polypeptide-polymer conjugates” means in the context of the present invention conjugates having a reduced immune response in humans and animals and/or a improved stability. As will be described further below the immune response is dependent on the way of challenge.
The present inventors have found that polypeptides, such as enzymes, may be made less immunogenic and/or allergenic by adding and/or removing one or more attachment groups on the surface of the parent polypeptide to be coupled to polymeric molecules.
When introducing pharmaceutical polypeptide directly into the circulatory system (i.e. bloodstream) the potential risk is an immunogenic response in the form of mainly IgG, IgA and/or IgM antibodies. In contrast hereto, industrial polypeptides, such as enzymes used as a functional ingredient in e.g. detergents, are not intended to enter the circulatory system. The potential risk in connection with industrial polypeptides is inhalation causing an allergenic response in the form of mainly IgE antibody formation.
Therefore, in connection with industrial polypeptides the potential risk is respiratory allergenicity caused by inhalation, intratracheal and intranasal presentation of polypeptides.
The main potential risk of pharmaceutical polypeptides is immunogenicity caused by, intradermal, intravenous, or subcutaneous presentation of the polypeptide.
It is to be understood that reducing the “immunogenicity” and reducing the “respiratory allergenicity” are two very different problems based on different routes of exposure and on two very different immunological mechanisms:
The term “immunogenicity” used in connection with the present invention may be referred to as allergic contact dermatitis in a clinical setting and is a cell mediated delayed immune response to chemicals that contact and penetrate the skin. This cell mediated reaction is also termed delayed contact hypersensitivity (type IV reaction according to Gell and Combs classification of immune mechanisms in tissue damage).
The term “allergenicity” or “respiratory allergenicity” is an immediate anaphylactic reaction (type I antibody-mediated reaction according to Gell and Combs) following inhalation of e.g. polypeptides.
According to the present invention it is possible to provide polypeptides with a reduced immune response and/or improved stability, which has a substantially retained residual activity.
The allergic and the immunogenic response are in one term, at least in the context of the present invention called the “immune response”.
In the first aspect the invention relates to a polypeptide-polymer conjugate having
a) one or more additional polymeric molecules coupled to the polypeptide having been modified in a manner to increase the number of attachment groups on the surface of the polypeptide in comparison to the number of attachment groups available on the corresponding parent polypeptide, and/or
b) one or more fewer polymeric molecules coupled to the polypeptide having been modified in a manner to decrease the number of attachment groups at or close to the functional site(s) of the polypeptide in comparison to the number of attachment groups available on the corresponding parent polypeptide.
The term “parent polypeptide” refers to the polypeptide to be modified by coupling to polymeric molecules. The parent polypeptide may be a naturally-occurring (or wild-type) polypeptide or may be a variant thereof prepared by any suitable means. For instance, the parent polypeptide may be a variant of a naturally-occurring polypeptide which has been modified by substitution, deletion or truncation of one or more amino acid residues or by addition or insertion of one or more amino acid residues to the amino acid sequence of a naturally-occurring polypeptide.
A “suitable attachment group” means in the context of the present invention any amino acid residue group on the surface of the polypeptide capable o
Olsen Arne Agerlin
Osten Claus von der
Roggen Erwin Ludo
Achutamurthy Ponnathapu
Lambiris Elias J.
Moore William W.
Novozymes A/S
LandOfFree
Modified enzymes having polymer conjugates does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Modified enzymes having polymer conjugates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified enzymes having polymer conjugates will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3032393