Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Reexamination Certificate
2006-09-26
2006-09-26
O'Hara, Eileen B. (Department: 1646)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
C424S085200
Reexamination Certificate
active
07112660
ABSTRACT:
The invention relates to methods for the stabilisation of cytokines and to cytokines generated by such methods. The methods of the invention involve mutating the amino acid sequence of a cytokine so as to remove solvent-exposed hydrophobic residues, and/or mutating the amino acid sequence of the cytokine so as to stabilise one or more secondary structure elements in the molecule. These steps have the effect of destabilsing intermediates that are formed during the folding process, relative to the stability of the cytokine in its naturally folded state, so increasing the yield of the cytokine as produced in vitro.
REFERENCES:
patent: 5596072 (1997-01-01), Culpepper et al.
Wang et al., Proc. Natl. Acad. Sci. USA, 94:1657-1662, 1997.
Wells, J.A. Biochemistry. 29(37):8509-8517, 1990.
Bowie et. al., Science 247: 1306-1310, 1990.
Domingues Helena
Oschkinat Hartmut
Peters Joerg
Serrano Luis
Alexander John B.
Bayer AG
Chandra Gyan
Conlin David G.
Edwards Angell Palmer & & Dodge LLP
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