Modified cyclodextrin glycosyltransferases for producing .gamma.

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435193, 536 232, 935 10, 935 14, C12N 910, C12N 1554, C12P 1918

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054749174

ABSTRACT:
A cyclodextrin glycosyltransferase (CGTase) which, in the conversion of starch or starch-like substrates to CD, produces .gamma.-CD in an increased amount, and whose protein sequence, in the region between amino acid position 180 and amino acid position 240, contains the amino acid sequence (SEQ ID NO: 1), where position 1 of the protein sequence is the beginning of the signal peptide of the CGTase and Xxx denotes a natural amino acid.

REFERENCES:
patent: 3812011 (1974-05-01), Okada et al.
patent: 4418144 (1983-11-01), Okada et al.
patent: 4808232 (1989-02-01), Beesley
patent: 4822874 (1989-04-01), Schmid et al.
Database WPI, Week 8813, Derwent Publications Ltd., London, GB; AN 88-088335 & JP-A-63039597 (Hayashibara Biochem), Feb. 20, 1988 (abstract).
Applied and Environmental Microbiology, vol. 58, No. 12, Dec. 1992, Am. Soc. Microbiol., Washington, D.C., US; pp. 4016-4025 S. Fujiwara et al. "Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the NH2-terminal region of the enzyme", FIG. 3,7.
Agric. Biol. Chem., vol. 54, No. 1, Jan. 1990, Tokyo, Japan, pp. 197-201, T. Kaneko et al., "Comparison of CD composition produced by chimeric CGTases".
Biotechn. Appl. Biochem., vol. 12, No. 4, Aug. 1990, Academic Press, N.Y., US, pp. 387-396, J. Hellman et al. "Effects of modifications at the C-terminus of cyclomaltodextrin glucanotransferase from Bacillus circulans var. alkalophilus on catalytic activity".
Database WPI, Week 9313, Derwent Publications Ltd., London, GB: AN 93-103608, & JP-A-5041985 (Oji Corn Starch Co. Ltd.), Feb. 23, 1993. (abstract).
Database WPi, Week 9151, Derwent Publications Ltd., London, GB, AN 91-373420, & JP-A-3251183 (Oji Corn Starch KK) 8. Nov. 1991. (abstract).
Biochemistry, vol. 31, No. 37, Sep. 22, 1992, Am. Chem. Soc. Washington, D.C., US, pp. 8740-8746, C. Klein et al. "Catalytic center of cyclodextrin glycosyltransferase derived from X-ray structure analysis combined with site directed mutagenesis".
J. Fermentation and Bioengineering, vol. 74, No. 6, 1992, Suita, Japan; pp. 345 to 351, N. Kitamoto et al. "Cloning and sequencing glucanotransferase from Bacillus sp. KC201".
Database WPI, Week 9339, Derwent Publications Ltd., London, GB, AN 93-308317 & JP-A-5219948 (Uozumi T) Aug. 31, 1993 (abstract).
Journal of Biotechnology, vol. 32, No. 3, 1994, Elsevier, Amsterdam, NL, pp. 283-288, K.-A. Sin et al. "Replacement of an amino acid residue of cyclodextrin glucanotransferase of Bacillus ohbensis doubles the production of gamma-cyclodextrin".
Database WPI, Week 9344, Derwent Publications Ltd., London, GB; AN 93-347473 & JP-A-5244945 (Nippon Shokuhin Kako KK), Sep. 24, 1993 (abstract).
J. Bacteriol. (1986) 166, pp. 1118-1122, Toshiya Takano et al. "Molecular Cloning, DNA-Nucleotide Sequencing, and Expression in Bacillus subtilis Cells of the Bacillus macerans Cyclodextrin Glucanotransferase Gene" is an English language reference.
Gene (1986) 47, pp. 269-277, Florian Binder, O. Huber and August Bock, "Cyclodextrin-glycosyltransferase from Klebsiella pneumoniae M5al: cloning, nucleotide sequence and expression" is an English language reference.
Appl. Microbiol. Biotechnol. (1990) 34, pp. 229-230, Hans Bender, "Highly homologous cyclodextrin glycosyltransferases from Bacillus circulans strain 8 and a strain of Bacillus licheniformis" is an English language reference.
Journal of General Microbiol. (1988) 134, pp. 97-105, Takahiro Kaneko et al. "Molecular Cloning and Nucleotide Sequence of the Cyclomaltodextrin Glucantransferase Gene from the Alkalophilic Bacillus sp. Strain No. 38-2" is an English language reference.
Proceedings of the 4th International Symposium on Cyclodextrins (1988), pp. 87-92, G. Schmid et al. "Selective Complexing Agents for the Production of .gamma.-Cyclodextrin" is an English language reference.
Appl. Microbiol. Biotechnol. (1987) 26, pp. 149-153, Kenji Kimura et al. "Molecular Cloning of the .beta.-Cyclodextrin Synthetase Gene form an alkalophilic Bacillus and its expression in Escherichia coli and Bacillus subtilis" is an English language literature.
Proceedings of the 4th International Symposium on Cyclodextrins (1988), pp. 71-76, G. Schmid et al., "Cloning and Nucleotide Sequence of a Cyclodextrin Glycosyltransferase Gene from the Alkalophilic Bacillus 1--1" is an English language reference.
Agric. Biol. Chem. (1986), 50, pp. 2161-2162, Takashi Kato and Koki Horikoshi "Cloning and Expression of the Bacillus subtilis No. 313 .gamma.-Cyclodextrin Forming CGTase Gene in Escherichia coli" is an English language reference.
Journal of Fermentation and Bioengineering, vol. 70, No. 3, 150-154, 1990, Yoshito Fujita et al., "Purification and Properties of Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6" is an English language reference.
Journal of Mol. Biol. (1991), 217, pp. 737-750, Claudio Klein and Georg E. Schulz, "Structure of Cyclodextrin Glycosyltransferase Refined at 2.0 .ANG. Resolution" is an English language reference.
Biochemistry (1992), 31, pp. 8740-8746, Claudio Klein et al., "Catalytic Center of Cyclodextrin Glycosyltransferase Derived from x-ray Structure Analysis Combined with Site-Directed Mutagenesis" is an English language reference.
J. H. Miller in (1972) Experiments in Molecular Genetics, Spring Harbor Laboratory, Cold SpriColdarbor, New York, is an English language reference.
Sanger et al. in Proc. Natl. Acad. Sci. USA, vol. 74, No. 12, pp. 5463-5467, Dec. 1977, "DNA sequencing with chain-terminating inhibotors" is an English language reference.
BioTechniques (1992) 13 (3), pp. 342-346, Bio Feedback, "Site Specific Mutagenesis of Almost Any Plasmid Using a PCR-Based Version of Unique Site" is an English language reference.
Technique--A Journal of Methods in Cell and Molecular Biology, vol. 1, No. 1 (Aug.), 1989, pp. 11-15, David W. Leung et al., "A Method for Ramdom Mutagenesis of a Defined DNA Segment using a Modified Polymerase Chain Reaction" is an English language reference.
F. M. Ausubel et al. (1987), Current Protocols in Molecular Biology, Greene Publishing Associates, John Wiley & Sons is an English language reference.
Promega 1992-1993 Catalogue 150, Biological Research Products, "Altered Sites in vitro Mutagenesis System" is an English language reference.
Ann. Rev. Genet. 1985, 19: pp. 423-462, Michael Smith, "In Vitro Mutagenesis" is an English language reference.
Journal of Fermentation and Bioengineering (1990), 70 (3), pp. 190-192, Kenji Tomita et al. "Some Factors Affecting the Formation of .gamma.-Cyclodextrin Using Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6" is an English language reference.
F. M. Ausubel in "Current Protocols in Molecular Biology", (1987) vol. 1; Greene Publishing Associates & Wiley--Interscience, New York is an English language reference.
T. Maniatis et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory (1982), New York, "Introduction of Plasmid and Bacteriophase .gamma.-DNA into Escherichia coli" is an English language reference.
T. Maniatis et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory (1982), New York, pp. 86-92, "Large Scale Isolation of Plasmid DNA" is an English language reference.
Nucl. Acids. Res. (1986) 14, pp. 9679-9698, K. L. Nakamaye et al., "Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis" is an English language reference.
Nucleic Acids Research, vol. 16, No. 3, 1988, J. R. Sayers et al. "5'-3'Exonucleases in phosphorothioate-based oligonucleotide--directed mutagenesis" is an English language reference.
T. Maniatis et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory (1982), New York, "Media and Antibiotics" is an English language reference.
Eur. J. Biochem. 191, pp. 177-185 (1990), FEBS 1990, A. Candussio et al. "Biochemical and Genetic Analysis of a Maltopentaose-producing amylase from an alkaliphilic Gram-positive bacterium" is an English language reference.
Mattsson et al., Applied Biochemistry & Biotechnol., vol. 30, 1991, pp. 17-28.
Sin et al., A

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