Modified cyclic nucleotide gated ion channels

Details Modified cyclic nucleotide gated ion channels Modified cyclic nucleotide gated ion channels

2006-11-16

2008-03-11

Saoud, Christine J. (Department: 1647)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S320100, C435S325000, C435S069100, C435S007200, C530S350000, C536S023500

Reexamination Certificate

active

07341836

ABSTRACT:
The present invention provides modified cyclic nucleotide gated (CNG) channels. In particularly preferred embodiments, the modified CNG channels exhibit increased sensitivity and specificity for cAMP, as compared to wild-type CNG channels. In additional embodiments, regulation by Ca2+-calmodulin has been removed in the modified CNG channels. Convenient optical methods for detecting changes in cAMP, taking advantage of the Ca2+permeability of the channel are also provided by the present invention. In addition, electrophysiological methods are further provided.

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