Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2006-01-12
2008-11-25
Campell, Bruce (Department: 1648)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C424S093450, C424S200100, C530S402000
Reexamination Certificate
active
07456011
ABSTRACT:
This disclosure relates to a modified version of cyanovirin-N protein and a transformedLactobacillusbacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformedLactobacillusbacterium. In addition, this disclosure describes two novel promoters originated fromL. jensenii, which are capable of directing a high level of gene expression inLactobacillusbacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modifiedLactobacillusbacterium containing the expression cassette, a method for recombinant gene expression inLactobacillusbacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.
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Essenmacher Kirsten
Liu Xiaowen
Simpson David A.
Xu Qiang
Blumel Benjamin P
Campell Bruce
Osel, Inc.
Townsend and Townsend / and Crew LLP
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