Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2006-04-18
2006-04-18
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C536S023710, C800S279000, C800S320300, C424S093200, C435S320100, C435S418000, C435S252300, C435S070100
Reexamination Certificate
active
07030295
ABSTRACT:
Compositions and methods for controlling plant pests are disclosed. In particular, novel nucleic acid sequences encoding modified Cry3A toxins having increased toxicity to corn rootworm are provided. By inserting a protease recognition site, such as cathepsin G, that is recognized by a gut protease of a target insect in at least one position of a Cry3A toxin a modified Cry3A toxin having significantly greater toxicity, particularly to western and northern corn rootworm is designed. Further, a method of making the modified Cry3A toxins and methods of using the modified cry3A nucleic acid sequences, for example in microorganisms to control insects or in transgenic plants to confer protection from insect damage, and a method of using the modified Cry3A toxins, and compositions and formulations comprising the modified Cry3A toxins, for example applying the modified Cry3A toxins or compositions or formulations to insect-infested areas, or to prophylactically treat insect-susceptible areas or plants to confer protection against the insect pests are disclosed.
REFERENCES:
patent: 5659123 (1997-08-01), Van Rie et al.
patent: 5763241 (1998-06-01), Fischhoff et al.
patent: 6013523 (2000-01-01), Adang et al.
patent: 6063597 (2000-05-01), Walters et al.
patent: WO99/00407 (1999-01-01), None
patent: WO 99/31248 (1999-06-01), None
Haider et al,Specificity of Bacillus ThuringiensisVar.Colmeri Insecticidal Delta-Endotoxin in Determined by Differential Proteolytic Processing of the Protoxin by Larval Gut Proteases European Journal of Biochemistry, vol. 156, No. 3 (May 1, 1986), pp. 531-540.
Smedley et al,Mutagenesis of three surface-exposed loops of a Bacillus thuringiensis insecticidal toxin reveals residues important for toxicity, receptor recognition and possibly membrane insertion Society for General Microbiology, vol. 142, No. Part 7 (July 1996), vol. 1617-1624.
Adang et al,The reconstruction and expression of a Bacillus thuringiensis cryIIIA gene in protoplasts and potato plants Plant Molecular Biology, vol. 21 (1993), pp. 1131-1145.
Carroll et al,Proteolytic processing of a coleopteran-specific δ-endotoxin produced by Bacillus thuringiensisvar.tenebrionis Biochemical Journal, vol. 26 (1989), pp. 99-105.
Carroll et al,Intramolecular Proteolytic Cleavage of Bacillus thuringiensis Cry3A δ-Endotoxin May Facilitate Its Coleopteran Toxicity Journal of Invertebrate Pathology, vol. 70 (1997) pp. 41-49.
Gazit et al,The structure and organization within the membrane of the helices composing the pore-forming domain of Bacillus thuringiensis δ-endotoxin are consistent with an “umbrella-like” structure of the pore Proceedings of the National Academy of Sciences, USA, vol. 95 (Oct. 1998), pp. 12289-12294.
Gazit, E. and Shai, Y.,The Assembly and Organization of the a 5 and a7 Helices from the Pore-forming Domain of Bacillus thuringiensis δ-endotoxin The Journal of Biological Chemistry, vol. 270, No. 6 (Feb. 10, 1995), pp. 2571-2578.
Gillikin et al,Partial Characterization of Digestive Tract Proteinases from Western Corn Rootworm Larvae, Diabrotica virgifera Archives of Insect Biochemistry and Physiology, vol. 19 (1992), pp. 285-298.
Li et al,Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5 Å resolution Nature, vol. 353 (Oct. 31, 1991), pp. 815-821.
Martinez-Ramirez, A.C. and Real M.D.,Proteolytic Processing of Bacillus thuringiensis CryIIIA Toxin and Specific Binding to Brush-Border Membrane Vesicles of Leptinotarsa decemlineata(Colorado Potato Beetle)Pesticide Biochemistry and Physiology, vol. 54 (1996), pp. 115-122, Article No. 0015.
McPherson et al,Characterization of the Cleopteran-Specific Protein Gene of Bacillus ThuringiensisVar.Tenebrionis Bio/Technology, vol. 6 (1998), pp. 61-66.
Oppert, B.,Protease Interactions With Bacillus thuringiensis Insecticidal Toxins Archives of Insect Biochemistry and Physiology, vol. 42 (1999), pp. 1-12.
Sekar et al.,Molecular cloning and characterization of the insecticidal crystal protein gene of Bacillus thuringiensisvar.tenebrionis Proceedings of the National Academy of Sciences, USA, vol. 84 (Oct. 1987), pp. 7036-7040.
Slaney et al,Mode of Action of Bacillus Thuringiensis Toxin CryIIIA: An Analysis of Toxicity in Leptinotarsa Decemlineata(Say)and Diabrotica Undecimpunctata Howardi Barber Insect Biochemistry Molecular Biology, vol. 22, No. 1 (1992), pp. 9-18.
Sutton et al,Synthetic cryIIIA gene from Bacillus thuringiensis improved for high expression in plants Transgenic Research, vol. 1 (1992), pp. 228-236.
Wu, S.J., Dean, D.H.,Functional Significance of Loops in The Receptor Binding Domain of Bacillus thuringiensis CryIIIA δ-Endotoxin Journal of Molecular Biology, vol. 255 (1996), pp. 628-640.
Wu et al,Enhanced toxicity of Bacillus thuringiensis Cry3A δ-Endotoxin in coleopterans by mutagenesis in a receptor binding loop Federation of European Biochemical Societies Letters, vol. 473 (2000), pp. 227-232.
Chen Eric
Stacy Cheryl
Kubelik Anne
Syngenta Participations (AG)
Syngenta Participations AG
LandOfFree
Modified Cry3A toxins and nucleic acid sequences coding... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Modified Cry3A toxins and nucleic acid sequences coding..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified Cry3A toxins and nucleic acid sequences coding... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3549593