Modified chondroitin synthase polypeptide and crystal thereof

Details Modified chondroitin synthase polypeptide and crystal thereof Modified chondroitin synthase polypeptide and crystal thereof

2011-04-19

2011-04-19

Nashed, Nashaat T (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S193000

Reexamination Certificate

active

07927837

ABSTRACT:
Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.

REFERENCES:
patent: 2003/0109693 (2003-06-01), Ninomiya et al.
patent: 1 283 259 (2003-02-01), None
patent: 2003 199583 (2003-07-01), None
patent: WO 2008/133350 (2008-11-01), None
Wiencek, J. M. Ann. Rev. Biomed. Eng. 1999, 1, 505-534.
DeAngelis et al. J. Biol. Chem. 2000, 275, 24124-24129.
T. Osawa, et al., “Crystal structure of chondroitin polymerase fromEscherichia colistrain K4 (K4CP) complexed with UDP-G1cUA and UDP”, Protein Data Bank, An Information Portal to Biological Macromolecular Structures, Database PDB {Online}, Retrieved from: http://www.rcsb. org/pdb/explore/explore.do?structureId=2Z86, Database accession No. 2Z86, XP002544108, Sep. 16, 2008, 1 page.
Ninomiya, T. et al., “Molecular Cloning and Characterization of Chondroitin Polymerase fromEscherichia coliStrain K4*”, The Journal of Biological Chemistry, vol. 277, No. 24, pp. 21567-21575, (2002).

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