Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues
Reexamination Certificate
2005-05-24
2005-05-24
Weber, Jon (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
C530S350000, C514S012200, C435S069700, C435S200000
Reexamination Certificate
active
06897285
ABSTRACT:
Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.
REFERENCES:
patent: 5187262 (1993-02-01), Raikhel et al.
patent: 5643758 (1997-07-01), Guan et al.
patent: 5834247 (1998-11-01), Comb et al.
patent: 5928917 (1999-07-01), Kilburn et al.
patent: 5962289 (1999-10-01), Kilburn et al.
patent: 6124117 (2000-09-01), Kilburn et al.
patent: 6174700 (2001-01-01), Haynes et al.
patent: WO 0047751 (2000-08-01), None
patent: WO 0157183 (2001-08-01), None
Yamagami et al. Biosci. Biotech. Biochem. 61(11): 1819-1825 1997.*
LaVallie and McCoy, Curr. Opin. Biotechnol., 6501-506 (1995).
Xu, et al., Methods Enzymol. 326:376-418 (2000).
Van Dyke, et al. Gene 111:99-104 (1992).
Smith and Johnson, Gene 67:31-40 (1998).
Watanabe, et al., J. Bacteriol. 176:4465-4472 (1994).
Hashimoto, et al. J. Bacteriol. 182:3045-3054 (2000).
Watanabe, et al. J. Bacteriol. 172:4017-4022 (1990).
Alam, et al. J. Ferment. Bioeng. 82:28-36 (1996).
Nagai, et al., Nature 309:810-812 (1984).
New England Catalog 2002/2003, p. 163.
Steel, et al. J. Immunol. 145:3917-3923 (1990).
Evans, et al., Biopolymers 51:333-342 (1999a).
Cantrell, et al. Proc. Natl. Acad. Sci, USA 82:6250-6254 (1985).
Mingsheng, et al., J. Biotechnol. 11:157-162 (1995).
Evans, et al. J. Biol. Chem. 274:18359-18363 (1999b).
Yamamoto, et al., Nature 319:230-234 (1986).
Doherty, et al., Proc. Natl. Acad. Sci. USA 96:10869-10874 (1999).
Chong, et al., Gene 192:271-281 (1997).
Studier, et al., Methods Enzymol. 185:60-89 (1990).
Bradford, Anal. Biochem., 72:248-254 (1976).
Ikegami et al., Solution Structure of the Chitin-binding Domain ofBacillus circulansWL-12 Chitinase A1, The Journal of Biological Chemistry, vol. 275, No. 18:13654-13661(2000).
Boller et al., Chitinase in Bean Leaves:Induction by Ethylene, Purification, Properties, and Possible Function, Planta, vol. 157:22-31 (1983).
Colussi Paul A.
Ferrandon Sebastien M.
Taron Christopher H.
Xu Ming-Qun
Desai Anand
New England Biolabs Inc.
Strimpel Harriet M.
Weber Jon
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