Modified Cdc14 phosphatase proteins and constructs for...

Details Modified Cdc14 phosphatase proteins and constructs for... Modified Cdc14 phosphatase proteins and constructs for...

2006-01-31

2006-01-31

Nashed, Nashaat T. (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S196000

Reexamination Certificate

active

06991915

ABSTRACT:
The present invention provides modified Cdc14 phosphatase proteins, and novel constructs for expression thereof, in which the sequence C-terminal to the catalytic domain has been deleted. This modification results in the expression of a single form of the enzyme, enabling purification of a homogeneous preparation of active enzyme at a high yield.

REFERENCES:
patent: 6331614 (2001-12-01), Wong et al.
Liwu Li et al.; “A Family of Putative Tumor Suppresors Is Structurally and Funtionally Conserved in Humans and Yeast;” The Journal of Biological Chemistry; vol. 272 No. 47, pp. 29403-29406; Nov. 21, 1997.
Liwu Li et al.; “The Human Cdc14 Phosphatases Interact with and Dephosphorylate the Tumor Suppressor Protein p53;” The Journal of Biological Chemistry vol. 275 No. 4 pp. 2410-2414; Jan. 28, 2000.
Alexander Wong et al. “Genomic Structure, Chromosonal Location, and Mutation Analysis of the Human CDC14A Gene;” Genomics vol. 59 pp. 248-251; Apr. 2, 1999.
Novagen; Product Catalog Dec. 1998 for their pET-30a-c(+) Vectors.
Genbank entry, GI: 15451928.
Genbank entry, GI: 15451929.

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