Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound
Patent
1997-02-28
1999-11-16
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing nitrogen-containing organic compound
435195, 435212, 435223, 435224, 435440, 530402, C12P 1302, C12N 914, C12N 948
Patent
active
059856270
ABSTRACT:
A method for transamidating a peptide substrate having a P.sub.1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S.sub.1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S.sub.1 ', S.sub.2 and/or S.sub.3 subsite to accommodate a specific peptide substrate.
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Breddam Klaus
Mortensen Uffe
Olesen Kjeld
S.o slashed.rensen Steen B.
Stennicke Henning
Carlsberg Laboratory
Wax Robert A.
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