Modified Bacillus thuringiensis insecticidal-crystal protein gen

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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4351723, 435419, 435418, C12N 514, C12N 1532, C12N 1582

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active

058773061

ABSTRACT:
A DNA fragment, encoding all or an insecticidally-effective part of a Bt crystal protein, is modified by changing A and T sequences to corresponding G and C sequences encoding the same amino acids, in a region having transcriptional activity less than 25% of the transcriptional activity of a region at the translation initiation site.

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Plant Physiology, vol. 85, 1987, pp. 1103-1109, Barton, K.A. et al., "Bacillus thuringiensis delta-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to Lepidopteran insects".
Nature, vol. 328, Jul. 2, 1987, pp. 33-37, Vaeck, M. et al., "Transgenic plants protected from insect attack".
Science, Sep. 27, 1987, vol. 237, pp. 1570, Lewin, R., "When Does Homology means Something Else?".
Barton KA, et al. "Bacillus thuringiensis gamma-endotoxin expressed in trangenic Nicotiana tabacum provides resistance to lepidopteran insects." Plant Physiol. 85: 1103-1109, 1987.
Perlak FJ, et al. "Modification of the coding sequence enhances plant expression of insect control protein genes."PNAS 88: 3324-3328, Apr. 1991.
Murray EE, et al. "Codon usage in plant genes". Nucl. Acids Res. 17: 477-498, 1989.
Hofte H, et al. "Structural and fuctional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715." Eur. J. Biochem. 161: 273-280, 1986.

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