Modified BACE

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252300, C435S252330, C435S320100, C435S325000, C435S410000, C536S023200

Reexamination Certificate

active

10372473

ABSTRACT:
The present invention relates to recombinant human BACE polypeptides. More particularly, the invention relates to recombinant human BACE polypeptides that have a modified amino acid sequence at position 33 of the BACE sequence, as well as to polynucleotides, expression vectors, host cells, and methods for producing the modified recombinant human BACE polypeptides.

REFERENCES:
patent: 5744346 (1998-04-01), Chrysler et al.
patent: 6319689 (2001-11-01), Powell et al.
patent: 6323326 (2001-11-01), Dorin et al.
patent: 9822597 (1998-05-01), None
patent: 0017369 (2000-03-01), None
patent: 0047618 (2000-08-01), None
patent: 0100663 (2001-01-01), None
patent: 0123533 (2001-04-01), None
patent: 0150829 (2001-07-01), None
Chou (2004) Journal of Proteome Research, vol. 3, No. 5, pp. 1069-1072.
Yan, Riqiang, et al;Membrane-anchored aspartyl protease with Alzheimer's disease β secretase activity; Nature, vol. 402, pp. 533-537, Dec. 2, 1999.
Vassar, Robert, et al; β-Secretase Cleavage of Alzheimer's Amyloid Precursor Protein by the Transmembrane Aspartic Protease BACE; Science, vol. 286, pp. 733-741, Oct. 22, 1999.
Sinha, Sukanto, et al;Purification and cloning of amyloid precursor protein β-secretase from human brain; Nature, vol. 402, 537-540, Dec. 2, 1999.
Mallender, William;Characterization of Recombinant, Soluble β-Secretase from an Insect Cell Expression System; Molecular Pharmacology; 59: 619-626, 2001.
Benjannet, Suzanne, et al;Post-translational Processingof β-Secretase (β-Amyloid-converting Enzyme) and it Ectodomain Shedding; The Journal of Biological Chemistry, vol. 276, No. 14, 10879-10887, Apr. 6, 2001.
Capell, Anja, et al;Maturation of Pro-peptide Cleavage of β -Secretase; The Journal of Biological Chemistry, vol. 275, No. 40, pp. 30849-30854, Oct. 6, 2000.
Charlwood, Joanne, et al;Characterization of the Glycosylation Profiles of Alzheimer's β-Secretase Protein Asp-2 Expressed in a Variety of Cell Lines; The Journal of Bioological Chemistry, vol. 276, No. 20, p. 16739-16748, May 18, 2001.
Ermolieff, Jacques, et al;Proteolytic Activation of Recombiant Pro-memapsin 2(Pro-β-secretase)Studied with new Fluorogenic Substrates; Biochemistry, 39, 12450-12456, 2000.
Haniu, Mitsuru, et al;Chracterization of Alzheimer's β-Secretase Protein BACE; The Journal of Biological Chemistry; vol. 275, No. 28, pp. 21099-21106, Jul. 14, 2000.
Hussain, Ishrut, et al;Identification of a Novel Aspartic Protease(Asp 2)as β-Secretase; Molecular and Cellular, 419-427, Neuroscience; 14, 1999.
Khan, Amir, et al;Molecular mechanisms for the convesion of zymogens to active proteolytic enzymes; Protein Science, 7:815-836, (1998).
Lin, Xinli, et al;Rearranging the domains of pepsinogen; Protein Science; 4:159-166, (1995).
Lin, Xin-Li, et al;Synthesis, Purification, and Active Site Mutagenesis of Recombinant Procine Pepsinogen; The Journal of Biological Chemistry; vol. 264, No. 8, pp. 4482-4489, Mar. 15, 1989.
Lin, Xinli, et al;Human aspartic proteas emeapsin 2 cleaves theβ-amyloid precursor protein; PNAS, vol. 97, pp. 1456-1460, Feb. 15, 2000.
Mildner, Ana, et al;Production of Chemokines CTAPIII and NAP/2 by Digestion of Recombinant Ubiquitin-CTAPIII with Yeast Ubiquitin C-Terminal Hydrolase and Human Immunodeficiency Virus Protease; Protein Expression and Purification vol. 16, 347-354, 1999.
Selkoe, D.J.;Cell Biology of theβ-Amyloid Precursor Protein and the Genetics of alzheimer's Disease; Cold Spring Harbor Symposia on Quantatative Biology, vol. LXI, pp. 587-596, 1996.
Selkoe, Dennis J.;Translating cell biology into therapeutic advances in Alzheimer's disease; Nature, vol. 399, pp. A23-A31, Jun. 24, 1999.
Thinakaran, Gopal, et al;Metabolism of the “Swedish” Amyloid Precursor Protein Variant in Neuro2a(N2a)Cells; the Journal of Biological Chemistry; vol. 271, No. 16, pp. 9390-9397, Apr. 19, 1996.
Inagami, T., et al; Prorenin, Biomed Res., vol. 1, pp. 456-475, 1980.
Schechter, Israel, et al;On the Size of the Active Site in Proteases. I Papain, Biochemical and Biophyscial Research Communications, vol. 27, No. 2, pp. 157-162, 1967.
Shi, Siao-Ping, et al;The Pro Domain of β-Secretase does not Confer Strict Zymogen-like Properties but Does Assit Proper Folding of the Protease Domain; vol. 276, No. 13, pp. 10366-10373, 2001.
Heinrikson, Robert L, et al;The Biochemistry and Molecular Biology of Recombinant Human Renin and Prorenin; Hypertension: Pathophysiology, Diagnosis and Management; Raven Press, Ltd., New York, pp. 1179-1196; 1990.
Hong, Lin, et al;Structure of the Protease Domain of Memapsin2(B-Secretase)Complexed with Inhibitor, Science Magazine, vol. 290, pp. 150-153, Oct. 6, 2000.
Selkoe, Dennis, J.;Alzheimer's Disease: Genes, Proteins and Therapy, Physiological Review, vol. 81, No. 2, pp. 741-766, Apr. 2001.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Modified BACE does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modified BACE, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified BACE will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3729416

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.