Modified avidin-biotin technique

Details Modified avidin-biotin technique Modified avidin-biotin technique

1992-07-20

1994-01-25

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 72, 435 792, 435960, 435962, 436501, 436548, G01N 33532, G01N 33577

Patent

active

052815218

ABSTRACT:
A method for preparing an antigen specific probe is provided by incubating a primary antigen specific monoclonal antibody with a biotinylated secondary antibody to produce a complex of the primary and secondary antibodies. The staining pattern produced by these probes reflects the specificity of the monoclonal antibody in the complex and the labeling of irrelevant, endogenous immunoglobulins is reduced substantially. This novel, indirect immunohistochemical method can be used to study normal and diseased tissues using a variety of monoclonal antibodies.

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