Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1997-09-30
2000-12-05
Achutamurthy, Ponnathapu
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 724, 435471, 530350, 530412, 530413, C12Q 170, G01N 3353, C07K 1436, C07K 114
Patent
active
061564931
ABSTRACT:
Streptavidin tetramers have at least one monomer containing an amino acid modification that produces a reduced binding affinity for biotin, a modified off-rate, a modified on-rate, or an altered binding enthalpy. Polynucleotides encoding the modified monomers are also provided. The modified streptavidin and chimeric streptavidin molecules are useful in methods of bioseparations and cell sorting, imaging, drug delivery, and diagnostics.
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Achutamurthy Ponnathapu
Moore William W.
University of Washington
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