Modified adeno-associated virus vector capable of expression fro

Details Modified adeno-associated virus vector capable of expression fro Modified adeno-associated virus vector capable of expression fro

1996-04-03

1999-02-02

Guzo, David

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 241, C07H 2104

Patent

active

058666966

ABSTRACT:
Described herein are constructions of recombinant DNA comprising modified adeno-associated virus (AAV) DNA sequences capable of functioning as a eukaryotic expression vector for expressing foreign DNA sequences using a novel transcription promoter comprising the termini of AAV DNA. It is shown that expression of a test reporter gene can be obtained from this vector in mammalian cells. It is further shown that this combination of vector and promoter can be used to introduce and express a human gene and correct a genetic defect in human cells resulting from malfunction of the mutant endogenous gene. Further, the vector can be used to correct the genetic defect by expressing a modified version of the human gene consisting of a fusion of part of the said gene and a synthetic sequence contained in the vector.

REFERENCES:
patent: 4797368 (1989-01-01), Carter et al.
patent: 5587308 (1996-12-01), Carter et al.
Tyssen et al., "Atlas of Invertebrate Viruses", Chapter 4, pp. 41-53, 1991.
Beaton et al., "Expression From The Adeno-Associated Virus P5 and P19 Promoters Is Negatively Regulated In Trans By The Rep Protein," Journal of Virology 63:4450-4454 (1989).
Riordan et al., "Identification Of The Cystic Fibrosis Gene: Cloning And Characterization Of Complimentary DNA," Science 245:1066-1073 (1989).
Collins et al. "Cystic Fibrosis: Molecular Biology And Therapeutic Implications" Science 256:774-779 (1992).
Wickens et al. "Role Of The Conserved AAVAAA Sequence: Four AAVAAA Point Mutants Prevent Messenger RNA 3'End Formation," Science 226:1045-1051 (1984).
Susan K. McLaughlin et al. Adeno-Associated Virus General Transduction Vectors: Analysis Of Proviral Structures, J. Virol. 62(6):1963-1973 (Jun. 1988).
Srivastava et al. "Construction Of A Recombinant Parvovirus B19: Adeno-Associated Virus 2 (AAV) DNA Inverted Terminal Repeats Are Functional In An AAV-B19 Hybrid Virus," Proc. Natl. Acad. Sci. 86:8078-8082 (Oct. 1989).
Rosenfeld et al., "In Vivo Transfer Of The Human Cystic Fibrosis Transmembrane Conductance Regulator Gene To The Airway Epithelium," Cell 68:143-155 (Jan. 10, 1992).

Affiliated with

Also associated with

Rating

Modified adeno-associated virus vector capable of expression fro does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modified adeno-associated virus vector capable of expression fro, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified adeno-associated virus vector capable of expression fro will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1118581