Modified adeno-associated virus vector capable of expression fro

Details Modified adeno-associated virus vector capable of expression fro Modified adeno-associated virus vector capable of expression fro

1999-01-21

2000-12-26

Guzo, David

Chemistry: molecular biology and microbiology

Vector, per se

536 231, 536 235, 536 241, C12N 15864, C07H 2104

Patent

active

061657816

ABSTRACT:
Described herein are constructions of recombinant DNA comprising modified adeno-associated virus (AAV) DNA sequences capable of functioning as a eukaryotic expression vector for expressing foreign DNA sequences using a novel transcription promoter comprising the termini of AAV DNA. It is shown that expression of a test reporter gene can be obtained from this vector in mammalian cells. It is further shown that this combination of vector and promoter can be used to introduce and express a human gene and correct a genetic defect in human cells resulting from malfunction of the mutant endogenous gene. Further, the vector can be used to correct the genetic defect by expressing a modified version of the human gene consisting of a fusion of part of the said gene and a synthetic sequence contained in the vector.

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