Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters carbohydrate production in the plant
Reexamination Certificate
1992-08-05
2001-09-25
Fox, David T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters carbohydrate production in the plant
C800S286000, C800S287000, C800S317200
Reexamination Certificate
active
06294713
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The subject invention relates to a process of reducing the accumulation of sugar in the tubers of potato plants.
2. Brief Description of Related Art
It is an object of the invention to effect a reduction in the amount of sucrose and/or free sugars derived from sucrose in potato tubers and/or one or more of sucrose, glucose or fructose formed from starch in potato tubers.
It has been suggested that sucrose phosphate synthase (SPS) (EC2.3.1.14) regulates the synthesis of sucrose in the leaves of higher plants (Stitt, N., Quick, P., Physiologia Plantarum 77 633-641, 1989) and that the enzyme occurs in tissues which synthesise sucrose (Stitt, M., Kaber, S., and Kerr, P., The Biochemistry of Plants 10 327-409, 1987, Academic Press).
Sucrose accumulates in potato tubers stored at low temperatures. Such accumulation of sugars at low temperature presents a significant problem to processors of potatoes. For example, producers of crisps and chips (french fries) have found that the presence of increased sugar tends to cause undue browning of the products during the frying process.
SUMMARY OF THE INVENTION
The subject invention provides a process for the preparation of a transgenic plant, which process comprises transforming a potato cell with a chimaeric gene comprising a suitable promoter for expression in a cell of a potato tuber and a sense or antisense genomic sequence for sugar reduction and regenerating a potato plant from the transformed cell, whereby in respect of the regenerated plant, as compared to a control plant not the product of said process, less of the sucrose imported into the tuber(s) from non-tuber parts of the plant is stored as sucrose and/or free sugar in the tuber(s) and/or less of one or more of sucrose, glucose or fructose is formed from starch stored in the tuber(s). The promoter should be such as to ensure that the sequence from the gene for sucrose phosphate synthase is expressed in the non-photosynthetic storage cells of the potato tuber.
The genome from which the sense or antisense sequence is derived may be, for example, potato, but the sequence may be derived from any other organism comprising a DNA sequence for sugar reduction which is sufficiently homologous to the endogenous sequence in the target potato plant.
REFERENCES:
patent: 0438904 A1 (1991-07-01), None
patent: WO 92/01782 (1992-02-01), None
patent: WO 92/16631 (1992-10-01), None
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J. Cell Biochem. Suppl. vol. 15A.
Blundy Keith Stuart
Burrell Michael Meyrick
Morris George Stephen
Thomas Christopher John Robert
Advanced Technologies (Cambridge) Limited
Fox David T.
Pennie & Edmonds LLP
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