Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Reexamination Certificate
2009-02-11
2011-11-15
Rao, Manjunath (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
C435S183000, C435S193000, C435S227000, C435S254800, C435S071100
Reexamination Certificate
active
08058053
ABSTRACT:
The present invention relates to methods and genetically engineered methylotrophic yeast strains for producing glycoproteins with mammalian-like glycosylation. The present invention also relates to vectors useful for generating methylotrophic yeast strains capable of producing glycoproteins with mammalian-like glycosylation. Glycoproteins produced from the genetically engineered methylotrophic yeast strains are also provided.
REFERENCES:
patent: 5135854 (1992-08-01), MacKay et al.
patent: 5705616 (1998-01-01), Lehle et al.
patent: 6803225 (2004-10-01), Contreras et al.
patent: 7029872 (2006-04-01), Gerngross
patent: 7252933 (2007-08-01), Contreras et al.
patent: 2002/0137134 (2002-09-01), Gerngross
patent: 2004/0038381 (2004-02-01), Contreras et al.
patent: 2008/0009037 (2008-01-01), Contreras et al.
patent: 0 314 096 (1989-05-01), None
patent: 0 548 012 (1993-06-01), None
patent: 0 582 244 (1994-02-01), None
patent: 1 211 310 (2002-06-01), None
patent: 8-336387 (1996-12-01), None
patent: WO 91/05057 (1991-04-01), None
patent: WO 92/09694 (1992-06-01), None
patent: WO 96/21038 (1996-07-01), None
patent: 01/14522 (2001-03-01), None
patent: WO 02/00856 (2002-01-01), None
patent: WO 02/00879 (2002-01-01), None
patent: 03/031464 (2003-04-01), None
patent: WO 03/506914 (2003-07-01), None
patent: WO 2004/074499 (2004-09-01), None
patent: WO 2005/100584 (2005-10-01), None
Maras, M., et al., “Molecular Cloning and Enzymatic Characterization of a Trichoderma reesei, 1,2—α-D-Mannosidase”, Journal of Biotechnology vol. 77, No. 2-3, pp. 255-263 (2000).
Bretthauer, R. K., et al., “Glycosylation ofPichia pastoris-derived Proteins”,Biotechnol. Appl Biochem, vol. 30, pp. 193-200 (1999).
Kukuruzinska, M. A., et al., “Protein Glycosylation in Yeast”,Ann. Rev. Biochem, vol. 56, pp. 915-944 (1987).
Chiba, Y., et al., “Production of Human Compatible High Mannose-Type (Man5GlcNAc2) Sugar Chains inSaccharomyces cerevisiae”, The Journal of Biological Chemistry, vol. 273, No. 41, pp. 26298-26304 (1998).
Maras, M., et al., In Vivo Synthesis of Complex N-Glycans by Expression of Human N-Acetylglucosaminyltransferase I in the Filamentous FungusTrichoderma reesei, FEBS Letters, vol. 452, pp. 365-370 (1999).
Nakanishi-Shindo, Y., et al., “Structure of theN-Linked Oligosaccharides That Show the Complete Loss of α-1,6-Polymannose Outer Chain fromoch1,och1mnn1, andoch1 mnnlalg3 Mutants ofSaccharomyces cerevisiae”, The Journal of Biological Chemistry, vol. 268, No. 35, pp. 26338-26345 (1993).
Martinet, W., et al., “Modification of the Protein Glycosylation Pathway in the Methylotrophic YeastPichia pastoris”, Biotechnology Letters, vol. 20, No. 12, pp. 1171-1177 (1998).
Maras, M., et al., “In vitro Conversion of the Carbohydrate Moiety of Fungal Glycoproteins to Mammalian-Type Oligosaccharides”,Eur. J. Biochem., vol. 249, pp. 701-707 (1997).
Laroy, W., et al., “Cloning ofTrypanosoma cruzi trans-Sialidase and Expression inPichia pastoris”, Protein Expression and Purification, vol. 20, pp. 389-393 (2000).
Inoue, T., et al., “Molecular Cloning and Nucleotide Sequence of the 1,2-a-D-Mannosidase Gene,msdS, fromAspergillus saitoiand Expression of the Gene in Yeast Cells”Biochimica et Biophysica Acta, vol. 1253, pp. 141-145 (1995).
Herscovics, A., et al., “Isolation of a Mouse Golgi Mannosidase cDNA, a Member of a Gene Family Conserved from Yeast to Mammals”,The Journal of Biological Chemistry, vol. 269, No. 13, pp. 9864-9871 (1994).
Lal, A., et al., “Isolation and Expression of Murine and Rabbit cDNAs Encoding an a1,2-Mannosidase Involved in the Processing of Asparagines-linked Oligosaccharides”,The Journal of Biological Chemistry, vol. 269, No. 13, pp. 9872-9881 (1994).
Trombetta, E.S., et al., “Endoplasmic Recticulum Glucosidase II is Composed of a Catalytic Subunit, Conserved from Yeast to Mammals, and a Tightly Bound Noncatalytic HDEL-containing Subunit”,The Journal of Biological Chemistry, vol. 271, No. 44, pp. 27509-27516 (1996).
Ngo, J.T., et al., “Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox”,The Protein Folding Problem and Tertiary Structure Prediction, pp. 491-495 (1994).
Rudinger, J., “Characteristics of the Amino Acids as Components of a Peptide Hormone Sequence”, In: Peptide Hormones (Parsons, J.A., Ed.), University Park Press, Baltimore, pp. 1-7 (1976).
Invitrogen Catalog, “Yeast Expression”, p. 22. (1998).
Cregg, J.M., et al., “High-Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast,Pichia pastoris”, Biotechnology, vol. 5, pp. 479-485 (1987).
Lehle, L., et al., “Glycoprotein Biosynthesis inSaccharomyces cerevisiae: ngd29, anN-glycosylation Mutant Allelic tooch1 having a Defect in the Initiation of Outer Chain Formation”,Federation of European Biochemical Societies, vol. 370, No. 1/2, pp. 41-45 (1995).
Yoko-o, T., et al., “Schizosaccharomyces pombe och1+ Encodes αa-1,6-mannosyltransferase that is Involved in Outer Chain Elongation of N-linked Oligosaccharides”,Federation of European Biochemical Societies, vol. 489, No. 1, pp. 75-80 (2001).
Lal, A., et al., “Substrate Specificities of Recombinant Murine Golgi α1,2-mannosidases IA and IB and Comparison with Endoplasmic Reticulum and Golgi Processing α1,2-mannosidases”,Glycobiology, vol. 8, No. 10, pp. 981-995 (1998).
Tremblay, L.O., et al., “Cloning and Expression of a Specific Human α1,2-mannosidase that Trims Man9GlcNAc2to Man8GlcNAc2Isomer B DuringN-glycan Biosynthesis”,Glycobiology, vol. 9, No. 10, pp. 1073-1078 (1999).
Gonzalez, D.S., et al., “Identification, Expression, and Characterization of a cDNA Encoding Human Endoplasmic Reticulum Mannosidase I, the Enzyme That Catalyzes the First Mannose Trimming Step in Mammalian Asn-linked Oligosaccharide Biosynthesis”,The Journal of Biological Chemistry, vol. 274, No. 30, pp. 21375-21386 (1999).
Kniskern, P.J., et al., “Characterization and Evaluation of a Recombinant Hepatitis B Vaccine Expressed in Yeast Defective for N-linked Hyperglycoslation”,Vaccine, vol. 11, No. 12, pp. 1021-1025 (1994).
Nakayama, K., et al., “OCH1 Encodes a Novel Membrane Bound Mannosyltransferase: Outer Chain Elongation of Asparagines-linked Oligosaccharides”,The EMBO Journal, vol. 11, No. 7, pp. 2511-2519 (1992).
Callewaert, N., et al., “Use of HDEL-TaggedTrichoderm reeseiMannosyl Oligosaccharide 1,2-α-D-Mannosidase for N-Glycan Engineering inPichia pastoris”, FEBS Letters, vol. 503, No. 2-3, pp. 173-178 (2001).
Chen, X., et al., “Carbohydrates in Transplantation”,Current Opinion in Chemical Biology, pp. 650-658 (1999).
Choi, B.K., et al., “Use of Combinatorial Genetic Libraries to Humanize N-linked Glycosylation in the YestPichia pastoris”, Proceedings of the National Academy of Sciences of the United States, vol. 100, No. 9, pp. 5022-5027 (2003).
Hamilton, S.R., et al., “Production of Complex Human Glycoproteins in Yeast”,Science, vol. 301, No. 5637, pp. 1244-1246 (2003).
Routier F. H. et al., “The glycosylation pattern of a humanized IgGI antibody (D1.3) expressed in CHO cells”,Glycoconjugate Journal 14: 201-207 (1997).
Kornfeld R. et al., “Assembly of Asparagine-Linked Oligosaccharides”,Ann. Rev. Biochem. 54: 631-664 (1985).
Malissard M. et al., “Expression of Functional Soluble Forms of Human β-1,4-Galactosyltransferase 1, α-2,6-Siayltransferase, and α-1,3-Fucosyltransferase VI in the Methylotrophic YeastPichia pastoris”, Biochemical and Biophysical Research Communications 267: 169-173 (2000).
Bencurova M. et al., “Expression of eukaryotic glycosyltransferases in the yeastPichia pastoris”, Biochimie 85: 413-422 (2003).
Schwientek T. et al., “
Callewaert Nico L. M.
Contreras Roland
Kaigorodov Vladimir
Vervecken Wouter
Gebreysesus Kagnew H
Rao Manjunath
Research Corporation Technologies Inc.
Scully , Scott, Murphy & Presser, P.C.
Universiteit Gent
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