Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1988-05-25
1990-08-21
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 84, 435822, 435823, 435 56, 4352521, C12P 1904, C12P 3318, C12R 101, C12N 122
Patent
active
049505971
ABSTRACT:
The present invention involves a process for screening for and isolating spontaneously occurring or induced cellulose II-producing microorganisms. The process comprises a series of steps in various embodiments. Initially, cellulose-producing microorganisms from a first culture are plated out on a nutrient agar plate. The nutrient agar plate is then incubated to facilitate formation of colonies from single microorganisms. Samples of liquid nutrient medium are then inoculated with microorganisms from colonies having a smooth configuration, as compared to the usual rough colony configuration. The inoculated samples are then aerobically incubated to facilitate microorganism proliferation and pellicle formation. From these incubated samples are selected microorganisms, which, after a cultivation period, have proliferated but not formed a pellicle. Said selected microorganisms produce cellulose II instead of the cellulose I produced by pellicle-forming organisms.
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Brown, Jr. R. Malcolm
Roberts Eric M.
Saxena Inder M.
Griffin Ronald W.
The University of Texas Board of Regents
Webber Pamela S.
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