Modification of animal lung surfactant for treating respiratory

Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Respiratory system

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514 21, 424422, A61K 3542

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active

061299340

ABSTRACT:
The present invention relates to the method of using a composition of matter that maintains the biophysical and physiological characteristics of natural surfactant, including the rapid and spontaneous adsorption of dilute suspensions to an air-liquid interface; the generation of low surface tension (<2.1 mN/m) at a surface area compression of 50% by the pulsating bubble method of Enhorning; and the restoration of greater than 75% of the lost volume of a surfactant deficient lung as determined by the method of Bermel. The material is produced by adding disaturated phospholipid to the extract of surface active material obtained by lavage of the air space of the lungs of animals. The material contains 90-96% phospholipid, 0-6% cholesterol, and 1-3% protein. Greater than 99% of the protein is lung surfactant apoproteins B & C. A sterile pharmaceutical preparation of the surface active material can be used for treating diseases caused by deficiency or dysfunction or natural lung surfactant.

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