Model for myeloid cell growth, differentiation and disease

Details Model for myeloid cell growth, differentiation and disease Model for myeloid cell growth, differentiation and disease

2005-04-26

2005-04-26

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S325000, C530S350000, C536S023400

Reexamination Certificate

active

06884589

ABSTRACT:
The invention is a genetic construct comprising the coding sequence for a hormone dependent E2a-Pbx1 protein. The invention is the use of the construct to generate hematopoietic cell lines arrested in differentiation upon exposure to the appropriate hormone. Upon removal of the hormone, cells undergo normal, synchronous differentiation providing a system for the study of normal differentiation. Expression of a number of oncogenes in the cells maintains the cells in an undifferentiated state, providing a model for the study the mechanisms of leukemia, and for the testing of pharmacological agents for the treatment and amelioration of the disease.

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