Model animal with overexpression of regucalcin

Details Model animal with overexpression of regucalcin Model animal with overexpression of regucalcin

2008-04-08

2008-04-08

Ton, Thaian N. (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

The nonhuman animal is a model for human disease

C800S014000

Reexamination Certificate

active

10804515

ABSTRACT:
The present invention provides an animal model which overexpresses regucalcin, a calcium-binding protein that is inherently expressed in the liver and the like of the higher animal, and which is characterized by a showing of bone pathology typified by osteoporosis. When regucalcin expression is lowered, it induces other physiological abnormalities. In the present invention, cDNA encoding the full length of regucalcin protein was cloned from a rat liver cDNA library, ORF was cut out, and introduced into an expression vector (pCXN2). The pCXN2 gene expression vector containing ORF cDNA was microinjected into the male pronucleus of a fertilized egg of rat which was subsequently transplanted into the uterine tube of a host rat to generate transgenic rats homozygous for regucalcin. The transgenic rats are characterized by remarkable bone pathology, morphologically as well as biochemically, and by significant suppression of body weight gain, and are therefore useful for screening of preventive and therapeutic agents related to bone diseases.

REFERENCES:
patent: 0 922 760 (1999-06-01), None
patent: 7-97399 (1995-04-01), None
patent: 7-123985 (1995-05-01), None
patent: 10-26623 (1998-01-01), None
patent: 11-9140 (1999-01-01), None
patent: WO 02/34041 (2002-05-01), None
Yamaguchi et al. (Jun. 24, 2002) J. Cell. Biochem 86:520-529.
Yamaguchi et al. (2002) Int. J. Mol. Med. 10:377-383.
Downs et al. (1999) Calcif. Tissue Int 64 :463-469.
Kolb et al. (1999) Gene 227:21-31.
Sigmund, C., Jun. 2000 (Arterioscler. Thromb. Vasc. Biol., p. 1425-1429).
Houdebine, L-M., 2002 (Journal of Biotechnology, vol. 98, p. 145-160).
Houdebine et al. (2000) Transgenic Research 9:305-320.
Murray (1999) Theriogenology 51:149-159.
Ebert et al Mol Endocrinol. 1988; 2(3): 277-83.
Sordet et al Joint Bone Spine. 2005; 72(6): 503-14.
Chenug et al Bone. 2006; 39(3): 470-6.
Mullins et al Nature, 1990, 344; 543-544.
Hammer et al. Journal of animal Science, 1986, 63, 269-278.
Mullins et al , EMBO, 1989, 8: 4065-4072.
Taurog, J. Immunology, 1988, 141: 4020-4023.
Hammer et al Cell, 1990, 63: 1099-1112.
Gibbs et al. Nature 428:493-521,2004.
Malakoff. Science 288: 248-253, 2000.
Xu et al. Nat Genet 20: 78-82, 1998.
Ferrari et al. Curr Opin Lipidol 16: 207-213, 2005.
Saftig et al. Proc Natl Acad Sci USA 95: 13453-13458, 1998.
Leheste et al. FASEB J 17:247-249, 2003.
David et al. J Bone Mineral Res 18: 1622-1631, 2003.
Aguirre et al. J Bone Mineral Res 21: 605-615, 2006.
Yamaguchi et al. J Cell Biochem 94: 794-803, 2005.
Haruhiko Inoue, et al., Senescence Marker Protein-30 (SMP30) Enhances The Calcium Efflux From Renal Tubular Epithelial Cells, Clin. Exp. Nephrol (1999) vol. 3, p. 261-267.
Terunobu Ishigami, et al., Regulatory Effects Of Senescence Marker Protein 30 On The Proliferation Of Hepatocytes, Pathology International (2001) vol. 51, p. 491-497.
Hiroyuki Misawa, et al., Suppression Of Cell Proliferation And Deoxyribonucleic Acid Synthesis In Cloned Rat Hepatoma H4-II-E Cells Overexpressing Regucalcin, Journal of Cellular Biochemistry (2002) vol. 84, p. 143-149.
Masayoshi Yamaguchi, The Role Of Regucalcin In Nuclear Regulation Of Regenerating Liver, Biochemical And Biophysical Research Communications (2000) vol. 276, p.

Affiliated with

Also associated with

Rating

Model animal with overexpression of regucalcin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Model animal with overexpression of regucalcin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Model animal with overexpression of regucalcin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3947175