Mobility-modified nucleobase polymers and methods of using same

Details Mobility-modified nucleobase polymers and methods of using same Mobility-modified nucleobase polymers and methods of using same

2011-03-01

2011-03-01

Staples, Mark (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S025300, C536S026600

Reexamination Certificate

active

07897338

ABSTRACT:
The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.

REFERENCES:
patent: 4914210 (1990-04-01), Levenson et al.
patent: 5185444 (1993-02-01), Summerton et al.
patent: 5470705 (1995-11-01), Grossman et al.
patent: 5470967 (1995-11-01), Huie et al.
patent: 5470974 (1995-11-01), Summerton et al.
patent: 5514543 (1996-05-01), Grossman et al.
patent: 5580732 (1996-12-01), Grossman et al.
patent: 5624800 (1997-04-01), Grossman et al.
patent: 5703222 (1997-12-01), Grossman et al.
patent: 5777096 (1998-07-01), Grossman et al.
patent: 5807682 (1998-09-01), Grossman et al.
patent: 6013785 (2000-01-01), Bruice et al.
patent: 6063569 (2000-05-01), Gildea et al.
patent: 6087325 (2000-07-01), Meers et al.
patent: 6143716 (2000-11-01), Meers et al.
patent: 6339069 (2002-01-01), Meers et al.
patent: 6395486 (2002-05-01), Grossman
patent: 6743905 (2004-06-01), Woo et al.
patent: 7074569 (2006-07-01), Woo et al.
patent: 2 443 122 (2003-10-01), None
patent: 1 379 698 (2009-08-01), None
patent: WO 93/20236 (1993-10-01), None
patent: WO 93/20239 (1993-10-01), None
patent: WO 02/083954 (2002-10-01), None
Stratagene catalog, 1998, p. 39.
Herdewijn et al., “Nucleotides (Part XXXII), Synthesis of 2′-5′ Connected Oligonucleotides. Prodrugs for Antiviral and Antitumoral Nucleosides” Helvetica Chimica Acta, vol. 72, No. 8, 1989, pp. 1739-1748.
W. Kuijpers et al., “A New Strategy for the Solid-Phase Synthesis of 5′-Thiolated Oligodeoxynucleotides” Tetrahedron, Elsevier Science Publishers, Amsterdam, NL, vol. 49, No. 47, Nov. 19, 1993, pp. 10931-10944.
B. Seelig et al., “Site-specific Modification of Enzymatically Synthesized RNA: Transcription Initiation and Diels-Alder Reaction”,Tetrahedron Letters, Elsevier, Amsterdam, NL, vol. 38, No. 44, Nov. 3, 1997, pp. 7729-7732.
B. Connolly, “Solid Phase 5′ Phosphorylation of Oligonucleotides” Tetrahedron Letters, vol. 28, No. 4, 1987, pp. 463-466.
L. Mizrakh et al., “Reactions of Alkyl Tetraalkylphosphorodiamidites with N-(hydroxyethyl)Acetamide” J. Gen. Chem. USSR, vol. 45, 1975, p. 2301.
J. Verheijen et al, “2′,5′-Oligoadenylate-Peptide Nucleic Acids (2-5A-PNAS) Activate RNASE L” Bioorganic & Medicinal Chemistry, Elsevier Science LTD, GB, vol. 7, 1999, pp. 449-455.
S. Rumney et al., “Structural Optimisation of Non-Nucleotide Loop Replacements for Duplex and Triplex DNAs” J. Am. Chem. Soc., vol. 117, 1995, pp. 5637-5646.
Supplementary European Search Report dated Mar. 14, 2007 cited in EP Application No. 02731376.6, 4 pages.
English version of Abstract for Japanese Patent Publication No. JP 4452445 B2, of Issue Date Apr. 21, 2010, based upon the Abstract for corresponding publication WO02/083954.
English translation of Abstract for Japanese Patent Publication No. JP2009240332 (A), published on Oct. 22, 2009.
Official Communication from the European Patent Office, dated Aug. 29, 2007, for EP Application No. 02 731 376.8.
Official Communication from the European Patent Office, dated Mar. 6, 2008, for EP Application No. 02 731 376.8.
English translation of the Japanese Patent Office's Notice of Reasons for Rejection for Application No. 2002-581693, dated Jan. 30, 2009.

Affiliated with

Also associated with

Rating

Mobility-modified nucleobase polymers and methods of using same does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mobility-modified nucleobase polymers and methods of using same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mobility-modified nucleobase polymers and methods of using same will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2629364